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Mok, Young Gun
유전체 교정 연구단
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Targeted A-to-G base editing of chloroplast DNA in plants

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Title
Targeted A-to-G base editing of chloroplast DNA in plants
Author(s)
Young Gun Mok; Sung Hyun Hong; Su Ji Bae; Sung-Ik Cho; Jin Soo Kim
Publication Date
2022-12
Journal
Nature Plants, v.8, no.12, pp.1378 - 1384
Publisher
Nature Research
Abstract
© 2022, The Author(s).Chloroplast DNA (cpDNA) encodes up to 315 (typically, 120–130) genes1, including those for essential components in photosystems I and II and the large subunit of RuBisCo, which catalyses CO2 fixation in plants. Targeted mutagenesis in cpDNA will be broadly useful for studying the functions of these genes in molecular detail and for developing crops and other plants with desired traits. Unfortunately, CRISPR–Cas9 and CRISPR-derived base editors, which enable targeted genetic modifications in nuclear DNA, are not suitable for organellar DNA editing2, owing to the difficulty of delivering guide RNA into organelles. CRISPR-free, protein-only base editors (including DddA-derived cytosine base editors3–8 and zinc finger deaminases9), originally developed for mitochondrial DNA editing in mammalian cells, can be used for C-to-T, rather than A-to-G, editing in cpDNA10–12. Here we show that heritable homoplasmic A-to-G edits can be induced in cpDNA, leading to phenotypic changes, using transcription activator-like effector-linked deaminases13.
URI
https://pr.ibs.re.kr/handle/8788114/12496
DOI
10.1038/s41477-022-01279-8
ISSN
2055-0278
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > 1. Journal Papers (저널논문)
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