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유전체교정연구단
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Targeted A-to-G base editing of chloroplast DNA in plants

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dc.contributor.authorYoung Gun Mok-
dc.contributor.authorSung Hyun Hong-
dc.contributor.authorSu Ji Bae-
dc.contributor.authorSung-Ik Cho-
dc.contributor.authorJin Soo Kim-
dc.date.accessioned2023-01-26T02:25:04Z-
dc.date.available2023-01-26T02:25:04Z-
dc.date.created2022-12-20-
dc.date.issued2022-12-
dc.identifier.issn2055-0278-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/12496-
dc.description.abstract© 2022, The Author(s).Chloroplast DNA (cpDNA) encodes up to 315 (typically, 120–130) genes1, including those for essential components in photosystems I and II and the large subunit of RuBisCo, which catalyses CO2 fixation in plants. Targeted mutagenesis in cpDNA will be broadly useful for studying the functions of these genes in molecular detail and for developing crops and other plants with desired traits. Unfortunately, CRISPR–Cas9 and CRISPR-derived base editors, which enable targeted genetic modifications in nuclear DNA, are not suitable for organellar DNA editing2, owing to the difficulty of delivering guide RNA into organelles. CRISPR-free, protein-only base editors (including DddA-derived cytosine base editors3–8 and zinc finger deaminases9), originally developed for mitochondrial DNA editing in mammalian cells, can be used for C-to-T, rather than A-to-G, editing in cpDNA10–12. Here we show that heritable homoplasmic A-to-G edits can be induced in cpDNA, leading to phenotypic changes, using transcription activator-like effector-linked deaminases13.-
dc.language영어-
dc.publisherNature Research-
dc.titleTargeted A-to-G base editing of chloroplast DNA in plants-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000903543300012-
dc.identifier.scopusid2-s2.0-85143271281-
dc.identifier.rimsid79499-
dc.contributor.affiliatedAuthorYoung Gun Mok-
dc.contributor.affiliatedAuthorSung Hyun Hong-
dc.contributor.affiliatedAuthorSu Ji Bae-
dc.contributor.affiliatedAuthorSung-Ik Cho-
dc.contributor.affiliatedAuthorJin Soo Kim-
dc.identifier.doi10.1038/s41477-022-01279-8-
dc.identifier.bibliographicCitationNature Plants, v.8, no.12, pp.1378 - 1384-
dc.relation.isPartOfNature Plants-
dc.citation.titleNature Plants-
dc.citation.volume8-
dc.citation.number12-
dc.citation.startPage1378-
dc.citation.endPage1384-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaPlant Sciences-
dc.relation.journalWebOfScienceCategoryPlant Sciences-
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > 1. Journal Papers (저널논문)
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