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Tissue clearing

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Title
Tissue clearing
Author(s)
Douglas S. Richardson; Webster Guan; Katsuhiko Matsumoto; Chenchen Pan; Kwanghun Chung; Ali Ertürk; Hiroki R. Ueda; Jeff W. Lichtman
Publication Date
2021-12
Journal
Nature Reviews Methods Primers, v.1, no.1
Publisher
Springer Nature
Abstract
Tissue clearing of gross anatomical samples was first described more than a century ago and has only recently found widespread use in the field of microscopy. This renaissance has been driven by the application of modern knowledge of optical physics and chemical engineering to the development of robust and reproducible clearing techniques, the arrival of new microscopes that can image large samples at cellular resolution and computing infrastructure able to store and analyse large volumes of data. Many biological relationships between structure and function require investigation in three dimensions, and tissue clearing therefore has the potential to enable broad discoveries in the biological sciences. Unfortunately, the current literature is complex and could confuse researchers looking to begin a clearing project. The goal of this Primer is to outline a modular approach to tissue clearing that allows a novice researcher to develop a customized clearing pipeline tailored to their tissue of interest. Furthermore, the Primer outlines the required imaging and computational infrastructure needed to perform tissue clearing at scale, gives an overview of current applications, discusses limitations and provides an outlook on future advances in the field. © 2021, Springer Nature Limited.
URI
https://pr.ibs.re.kr/handle/8788114/12212
DOI
10.1038/s43586-021-00080-9
ISSN
2662-8449
Appears in Collections:
Center for Nanomedicine (나노의학 연구단) > 1. Journal Papers (저널논문)
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