BROWSE

Related Scientist

Researcher

RNA 연구단
RNA 연구단
more info

TALEN-based knockout library for human microRNAs

Cited 44 time in webofscience Cited 0 time in scopus
784 Viewed 44 Downloaded
Title
TALEN-based knockout library for human microRNAs
Author(s)
Young-Kook Kim; Gabbine Wee; Joha Park; Jongkyu Kim; Daehyun Baek; Jin-Soo Kim; V Narry Kim
Publication Date
2013-12
Journal
NATURE STRUCTURAL & MOLECULAR BIOLOGY, v.20, no.12, pp.1458 - 1464
Publisher
NATURE PUBLISHING GROUP
Abstract
Various technical tools have been developed to probe the functions of microRNAs (miRNAs), yet their application has been limited by low efficacy and specificity. To overcome the limitations, we used transcription activator-like effector nucleases (TALENs) to knock out human miRNA genes. We designed and produced a library of 540 pairs of TALENs for 274 miRNA loci, focusing on potentially important miRNAs. The knockout procedure takes only 2-4 weeks and can be applied to any cell type. As a case study, we generated knockout cells for two related miRNAs, miR-141 and miR-200c, which belong to the highly conserved miR-200 family. Interestingly, miR-141 and miR-200c, despite their overall similarity, suppress largely nonoverlapping groups of targets, thus suggesting that functional miRNA-target interaction requires strict seed-pairing. Our study illustrates the potency of TALEN technology and provides useful resources for miRNA research.
URI
https://pr.ibs.re.kr/handle/8788114/1190
ISSN
1545-9993
Appears in Collections:
Center for RNA Research(RNA 연구단) > Journal Papers (저널논문)
Center for Genome Engineering(유전체 교정 연구단) > Journal Papers (저널논문)
Files in This Item:
2-6.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse