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나노물질 및 화학반응 연구단
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Conformational substates of myoglobin intermediate resolved by picosecond X-ray solution scattering

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Title
Conformational substates of myoglobin intermediate resolved by picosecond X-ray solution scattering
Author(s)
Key Young Oang; Jong Goo Kim; Cheolhee Yang; Tae Wu Kim; Youngmin Kim; Kyung Hwan Kim; Kim J.; HyotCherl Ihee
Publication Date
2014-03
Journal
JOURNAL OF PHYSICAL CHEMISTRY LETTERS, v.5, no.5, pp.804 - 808
Publisher
AMER CHEMICAL SOC
Abstract
Conformational substates of proteins are generally considered to play important roles in regulating protein functions, but an understanding of how they influence the structural dynamics and functions of the proteins has been elusive. Here, we investigate the structural dynamics of sperm whale myoglobin associated with the conformational substates using picosecond X-ray solution scattering. By applying kinetic analysis considering all of the plausible candidate models, we establish a kinetic model for the entire cycle of the protein transition in a wide time range from 100 ps to 10 ms. Four structurally distinct intermediates are formed during the cycle, and most importantly, the transition from the first intermediate to the second one (B → C) occurs biphasically. We attribute the biphasic kinetics to the involvement of two conformational substates of the first intermediate, which are generated by the interplay between the distal histidine and the photodissociated CO. © 2014 American Chemical Society.
URI
https://pr.ibs.re.kr/handle/8788114/1176
ISSN
1948-7185
Appears in Collections:
Center for Nanomaterials and Chemical Reactions(나노물질 및 화학반응 연구단) > Journal Papers (저널논문)
Files in This Item:
288_Journal of Physical Chemistry Letters_5_ 804-808_2014.pdfDownload

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