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나노의학 연구단
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Intestinal extracellular matrix hydrogels to generate intestinal organoids for translational applications

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Title
Intestinal extracellular matrix hydrogels to generate intestinal organoids for translational applications
Author(s)
Suran Kim; Yi Sun Choi; Jung Seung Lee; Sung-Hyun Jo; Yun-Gon Kim; Seung-Woo Cho
Publication Date
2022-03
Journal
Journal of Industrial and Engineering Chemistry, v.107, pp.155 - 164
Publisher
Korean Society of Industrial Engineering Chemistry
Abstract
© 2021 The Korean Society of Industrial and Engineering ChemistryIntestinal organoids are emerging tools in intestinal disease modeling and regeneration. Matrigel, a basement membrane matrix extracted from mouse sarcoma, is the gold standard material for intestinal organoid culture. Yet, the tumor origin of this matrix does not effectively provide organoids with native intestine-like microenvironments. To overcome this limitation, we demonstrate a decellularized small intestine-derived hydrogel reinforced with three extracellular matrix (ECM) proteins that are essential for intestinal development. To identify optimal conditions for intestinal organoid culture, we optimized the concentrations of the ECM hydrogel derived from decellularized intestine and three ECM proteins. The intestinal organoids grown in the established ECM hydrogels have shown similarity to those grown in Matrigel in terms of organoid forming efficiency, size, and gene expression patterns of intestine-specific markers. Our intestine-mimetic hydrogel could increase the feasibility of intestinal organoids for more clinically relevant applications in disease modeling and regenerative medicine.
URI
https://pr.ibs.re.kr/handle/8788114/11569
DOI
10.1016/j.jiec.2021.11.044
ISSN
1226-086X
Appears in Collections:
Center for Nanomedicine (나노의학 연구단) > 1. Journal Papers (저널논문)
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