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첨단연성물질연구단
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Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

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dc.contributor.authorKim, Chi-Ju-
dc.contributor.authorKuczler, Morgan D.-
dc.contributor.authorDong, Liang-
dc.contributor.authorJunyoung Kim-
dc.contributor.authorAmend, Sarah R.-
dc.contributor.authorYoon-Kyoung Cho-
dc.contributor.authorPienta, Kenneth J.-
dc.date.accessioned2022-04-07T04:55:17Z-
dc.date.available2022-04-07T04:55:17Z-
dc.date.created2022-03-15-
dc.date.issued2022-02-
dc.identifier.issn1940-087X-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/11366-
dc.description.abstract© 2022 JoVE Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.There is a need for practical assays to visualize and quantify the cells' extracellular vesicle (EV) uptake. EV uptake plays a role in intercellular communication in various research fields; cancer biology, neuroscience, and drug delivery. Many EV uptake assays have been reported in the literature; however, there is a lack of practical, detailed experimental methodology. EV uptake can be assessed by fluorescently labeling EVs to detect their location within cells. Distinguishing between internalized EVs in cells and the superficial EVs on cells is difficult, yet critical, to accurately determine the EV uptake. Therefore, an assay that efficiently quantifies EV uptake through three-dimensional (3D) fluorescence confocal microscopy is proposed in this work. Fluorescently labeled EVs were prepared using a nano-filtration-based microfluidic device, visualized by 3D confocal microscopy, and then analyzed through advanced image-processing software. The protocol provides a robust methodology for analyzing EVs on a cellular level and a practical approach for efficient analysis.-
dc.language영어-
dc.publisherMYJoVE Corporation-
dc.titleExtracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000802505600020-
dc.identifier.scopusid2-s2.0-85125385002-
dc.identifier.rimsid77859-
dc.contributor.affiliatedAuthorJunyoung Kim-
dc.contributor.affiliatedAuthorYoon-Kyoung Cho-
dc.identifier.doi10.3791/62836-
dc.identifier.bibliographicCitationJournal of Visualized Experiments, v.2022, no.180-
dc.relation.isPartOfJournal of Visualized Experiments-
dc.citation.titleJournal of Visualized Experiments-
dc.citation.volume2022-
dc.citation.number180-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaScience & Technology - Other Topics-
dc.relation.journalWebOfScienceCategoryMultidisciplinary Sciences-
dc.subject.keywordPlusEXOSOME ISOLATION-
dc.subject.keywordPlusWHOLE-BLOOD-
dc.subject.keywordPlusCELLS-
dc.subject.keywordPlusPROGRESS-
Appears in Collections:
Center for Soft and Living Matter(첨단연성물질 연구단) > 1. Journal Papers (저널논문)
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