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Tissue-specific DNA damage response in Mouse Whole-body irradiation

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Title
Tissue-specific DNA damage response in Mouse Whole-body irradiation
Author(s)
Seon-Gyeong Lee; Namwoo Kim; Park, In Bae; Park, Jun Hong; Kyungjae Myung
Publication Date
2022-01
Journal
Molecular and Cellular Toxicology, v.18, no.1, pp.131 - 139
Publisher
Springer Verlag
Abstract
© 2021, The Author(s).Background: Genomic instability is a hallmark of various cancers, and DNA repair is an essential process for maintaining genomic integrity. Mammalian cells have developed various DNA repair mechanisms in response to DNA damage. Compared to the cellular response to DNA damage, the in vivo DNA damage response (DDR) of specific tissues has not been studied extensively. Objective: In this study, mice were exposed to whole-body gamma (γ)-irradiation to evaluate the specific DDR of various tissues. We treated male C57BL6/J mice with γ-irradiation at different doses, and the DDR protein levels in different tissues were analyzed. Results: The level of gamma-H2A histone family member X (γH2AX) increased in most organs after exposure to γ-irradiation. In particular, the liver, lung, and kidney tissues showed higher γH2AX induction upon DNA damage, compared to that in the brain, muscle, and testis tissues. RAD51 was highly expressed in the testis, irrespective of irradiation. The levels of proliferating cell nuclear antigen (PCNA) and ubiquitinated PCNA increased in lung tissues upon irradiation, suggesting that the post-replication repair may mainly operate in the lungs in response to γ-irradiation. Conclusion: These results suggest that each tissue has a preferable repair mechanism in response to γ-irradiation. Therefore, the understanding and application of tissue-specific DNA damage responses could improve the clinical approach of radiotherapy for treating specific cancers.
URI
https://pr.ibs.re.kr/handle/8788114/11114
DOI
10.1007/s13273-021-00195-w
ISSN
1738-642X
Appears in Collections:
Center for Genomic Integrity(유전체 항상성 연구단) > 1. Journal Papers (저널논문)
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