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Epitope-preserving magnified analysis of proteome (eMAP)

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Title
Epitope-preserving magnified analysis of proteome (eMAP)
Author(s)
Joha Park; Sarim Khan; Dae Hee Yun; Taeyun Ku; Katherine L. Villa; Jiachen E. Lee; Qiangge Zhang; Juhyuk Park; Guoping Feng; Elly Nedivi; Kwanghun Chung
Publication Date
2021-11
Journal
Science Advances, v.7, no.46
Publisher
American Association for the Advancement of Science
Abstract
Copyright © 2021 The Authors.Synthetic tissue-hydrogel methods have enabled superresolution investigation of biological systems using diffraction-limited microscopy. However, chemical modification by fixatives can cause loss of antigenicity, limiting molecular interrogation of the tissue gel. Here, we present epitope-preserving magnified analysis of proteome (eMAP) that uses purely physical tissue-gel hybridization to minimize the loss of antigenicity while allowing permanent anchoring of biomolecules. We achieved success rates of 96% and 94% with synaptic antibodies for mouse and marmoset brains, respectively. Maximal preservation of antigenicity allows imaging of nanoscopic architectures in 1000-fold expanded tissues without additional signal amplification. eMAP-processed tissue gel can endure repeated staining and destaining without epitope loss or structural damage, enabling highly multiplexed proteomic analysis. We demonstrated the utility of eMAP as a nanoscopic proteomic interrogation tool by investigating molecular heterogeneity in inhibitory synapses in the mouse brain neocortex and characterizing the spatial distributions of synaptic proteins within synapses in mouse and marmoset brains.
URI
https://pr.ibs.re.kr/handle/8788114/11053
DOI
10.1126/sciadv.abf6589
ISSN
2375-2548
Appears in Collections:
Center for Nanomedicine (나노의학 연구단) > 1. Journal Papers (저널논문)
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