BROWSE

Related Scientist

RNA's photo.

RNA
RNA 연구단
more info

ITEM VIEW & DOWNLOAD

A quantitative map of human primary microRNA processing sites

Cited 0 time in webofscience Cited 0 time in scopus
11 Viewed 0 Downloaded
Title
A quantitative map of human primary microRNA processing sites
Author(s)
Kijun Kim; S. Chan Baek; Young-Yoon Lee; Carolien Bastiaanssen; Jeesoo Kim; Haedong Kim; V. Narry Kim
Publication Date
2021-08
Journal
Molecular Cell, v.81, no.16, pp.3422 - 3439.e11
Publisher
Cell Press
Abstract
© 2021 Elsevier Inc.Maturation of canonical microRNA (miRNA) is initiated by DROSHA that cleaves the primary transcript (pri-miRNA). More than 1,800 miRNA loci are annotated in humans, but it remains largely unknown whether and at which sites pri-miRNAs are cleaved by DROSHA. Here, we performed in vitro processing on a full set of human pri-miRNAs (miRBase version 21) followed by sequencing. This comprehensive profiling enabled us to classify miRNAs on the basis of DROSHA dependence and map their cleavage sites with respective processing efficiency measures. Only 758 pri-miRNAs are confidently processed by DROSHA, while the majority may be non-canonical or false entries. Analyses of the DROSHA-dependent pri-miRNAs show key cis-elements for processing. We observe widespread alternative processing and unproductive cleavage events such as “nick” or “inverse” processing. SRSF3 is a broad-acting auxiliary factor modulating alternative processing and suppressing unproductive processing. The profiling data and methods developed in this study will allow systematic analyses of miRNA regulation.
URI
https://pr.ibs.re.kr/handle/8788114/10549
DOI
10.1016/j.molcel.2021.07.002
ISSN
1097-2765
Appears in Collections:
Center for RNA Research(RNA 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
There are no files associated with this item.

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse