BROWSE

Related Scientist

RNA's photo.

RNA
RNA 연구단
more info

ITEM VIEW & DOWNLOAD

A quantitative map of human primary microRNA processing sites

DC Field Value Language
dc.contributor.authorKijun Kim-
dc.contributor.authorS. Chan Baek-
dc.contributor.authorYoung-Yoon Lee-
dc.contributor.authorCarolien Bastiaanssen-
dc.contributor.authorJeesoo Kim-
dc.contributor.authorHaedong Kim-
dc.contributor.authorV. Narry Kim-
dc.date.accessioned2021-11-02T05:30:10Z-
dc.date.available2021-11-02T05:30:10Z-
dc.date.created2021-11-01-
dc.date.issued2021-08-19-
dc.identifier.issn1097-2765-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/10549-
dc.description.abstract© 2021 Elsevier Inc.Maturation of canonical microRNA (miRNA) is initiated by DROSHA that cleaves the primary transcript (pri-miRNA). More than 1,800 miRNA loci are annotated in humans, but it remains largely unknown whether and at which sites pri-miRNAs are cleaved by DROSHA. Here, we performed in vitro processing on a full set of human pri-miRNAs (miRBase version 21) followed by sequencing. This comprehensive profiling enabled us to classify miRNAs on the basis of DROSHA dependence and map their cleavage sites with respective processing efficiency measures. Only 758 pri-miRNAs are confidently processed by DROSHA, while the majority may be non-canonical or false entries. Analyses of the DROSHA-dependent pri-miRNAs show key cis-elements for processing. We observe widespread alternative processing and unproductive cleavage events such as “nick” or “inverse” processing. SRSF3 is a broad-acting auxiliary factor modulating alternative processing and suppressing unproductive processing. The profiling data and methods developed in this study will allow systematic analyses of miRNA regulation.-
dc.language영어-
dc.publisherCell Press-
dc.titleA quantitative map of human primary microRNA processing sites-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000688617400004-
dc.identifier.scopusid2-s2.0-85112555085-
dc.identifier.rimsid76523-
dc.contributor.affiliatedAuthorKijun Kim-
dc.contributor.affiliatedAuthorS. Chan Baek-
dc.contributor.affiliatedAuthorYoung-Yoon Lee-
dc.contributor.affiliatedAuthorCarolien Bastiaanssen-
dc.contributor.affiliatedAuthorJeesoo Kim-
dc.contributor.affiliatedAuthorHaedong Kim-
dc.contributor.affiliatedAuthorV. Narry Kim-
dc.identifier.doi10.1016/j.molcel.2021.07.002-
dc.identifier.bibliographicCitationMolecular Cell, v.81, no.16, pp.3422 - 3439-
dc.relation.isPartOfMolecular Cell-
dc.citation.titleMolecular Cell-
dc.citation.volume81-
dc.citation.number16-
dc.citation.startPage3422-
dc.citation.endPage3439-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaBiochemistry & Molecular Biology-
dc.relation.journalResearchAreaCell Biology-
dc.relation.journalWebOfScienceCategoryBiochemistry & Molecular Biology-
dc.relation.journalWebOfScienceCategoryCell Biology-
dc.subject.keywordPlusMATURATION-
dc.subject.keywordPlusCOMPLEX-
dc.subject.keywordPlusRECOGNITION-
dc.subject.keywordPlusRNA-BINDING PROTEINS-
dc.subject.keywordPlusDROSHA CLEAVAGE-
dc.subject.keywordPlusDIFFERENTIAL EXPRESSION-
dc.subject.keywordPlusMIRNA BIOGENESIS-
dc.subject.keywordPlusMOLECULAR-BASIS-
dc.subject.keywordPlusHUMAN DICER-
dc.subject.keywordPlusMICROPROCESSOR-
dc.subject.keywordAuthorDGCR8-
dc.subject.keywordAuthorDROSHA-
dc.subject.keywordAuthorMicroprocessor-
dc.subject.keywordAuthormicroRNA-
dc.subject.keywordAuthorpri-miRNA processing-
dc.subject.keywordAuthorSRSF3-
Appears in Collections:
Center for RNA Research(RNA 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
There are no files associated with this item.

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse