BROWSE

Related Scientist

CNIR's photo.

CNIR
뇌과학 이미징 연구단
more info

ITEM VIEW & DOWNLOAD

Impact of the conjugation of antibodies to the surfaces of polymer nanoparticles on the immune cell targeting abilities

Cited 0 time in webofscience Cited 0 time in scopus
11 Viewed 0 Downloaded
Title
Impact of the conjugation of antibodies to the surfaces of polymer nanoparticles on the immune cell targeting abilities
Author(s)
Lee, Na Kyeong; Wang, Chi-Pin James; Lim, Jaesung; Park, Wooram; Kwon, Ho-Keun; Kim, Se-Na; Kim, Tae-Hyung; Chun Gwon Park
Publication Date
2021-12
Journal
Nano Convergence, v.8, no.1
Publisher
Korea Nano Technology Research Society
Abstract
© 2021, The Author(s).Antibodies have been widely used to provide targeting ability and to enhance bioactivity owing to their high specificity, availability, and diversity. Recent advances in biotechnology and nanotechnology permit site-specific engineering of antibodies and their conjugation to the surfaces of nanoparticles (NPs) in various orientations through chemical conjugations and physical adhesions. This study proposes the conjugation of poly(lactic-co-glycolic acid) (PLGA) NPs with antibodies by using two distinct methods, followed by a comparison between the cell-targeting efficiencies of both techniques. Full-length antibodies were conjugated to the PLGA-poly(ethylene glycol)-carboxylic acid (PLGA-PEG-COOH) NPs through the conventional carbodiimide coupling reaction, and f(ab′)2 antibody fragments were conjugated to the PLGA-poly(ethylene glycol)-maleimide(PLGA-PEG-Mal) NPs through interactions between the f(ab′)2 fragment thiol groups and the maleimide located on the nanoparticle surface. The results demonstrate that the PLGA nanoparticles conjugated with the f(ab′)2 antibody fragments had a higher targeting efficiency in vitro and in vivo than that of the PLGA nanoparticles conjugated with the full-length antibodies. The results of this study can be built upon to design a delivery technique for drugs through biocompatible nanoparticles.
URI
https://pr.ibs.re.kr/handle/8788114/10539
DOI
10.1186/s40580-021-00274-7
ISSN
2196-5404
Appears in Collections:
Center for Neuroscience Imaging Research (뇌과학 이미징 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
There are no files associated with this item.

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse