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Khriezhanuo, Nakhro
유전체 항상성 연구단
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Large-scale generation and phenotypic characterization of zebrafish CRISPR mutants of DNA repair genes

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Title
Large-scale generation and phenotypic characterization of zebrafish CRISPR mutants of DNA repair genes
Author(s)
Shin, Unbeom; Khriezhanuo Nakhro; Chang-Kyu Oh; Carrington, Blake; HeaIn Song; Varshney, Gaurav K.; Kim, Yeongjae; Hyemin Song; Sangeun Jeon; Robbins, Gabrielle; Kim, Sangin; Suhyeon Yoon; Yong Jun Choi; Kim, Yoo Jung; Burgess, Shawn; Sukhyun Kang; Sood, Raman; Yoonsung Lee; Kyungjae Myung
Publication Date
2021-11
Journal
DNA Repair, v.107
Publisher
Elsevier B.V.
Abstract
© 2021A systematic knowledge of the roles of DNA repair genes at the level of the organism has been limited due to the lack of appropriate experimental approaches using animal model systems. Zebrafish has become a powerful vertebrate genetic model system with availability due to the ease of genome editing and large-scale phenotype screening. Here, we generated zebrafish mutants for 32 DNA repair and replication genes through multiplexed CRISPR/Cas9-mediated mutagenesis. Large-scale phenotypic characterization of our mutant collection revealed that three genes (atad5a, ddb1, pcna) are essential for proper embryonic development and hematopoiesis; seven genes (apex1, atrip, ino80, mre11a, shfm1, telo2, wrn) are required for growth and development during juvenile stage and six genes (blm, brca2, fanci, rad51, rad54l, rtel1) play critical roles in sex development. Furthermore, mutation in six genes (atad5a, brca2, polk, rad51, shfm1, xrcc1) displayed hypersensitivity to DNA damage agents. Our zebrafish mutant collection provides a unique resource for understanding of the roles of DNA repair genes at the organismal level.
URI
https://pr.ibs.re.kr/handle/8788114/10415
DOI
10.1016/j.dnarep.2021.103173
ISSN
1568-7864
Appears in Collections:
Center for Genomic Integrity(유전체 항상성 연구단) > 1. Journal Papers (저널논문)
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