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Khriezhanuo, Nakhro
유전체 항상성 연구단
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Large-scale generation and phenotypic characterization of zebrafish CRISPR mutants of DNA repair genes

DC Field Value Language
dc.contributor.authorShin, Unbeom-
dc.contributor.authorKhriezhanuo Nakhro-
dc.contributor.authorChang-Kyu Oh-
dc.contributor.authorCarrington, Blake-
dc.contributor.authorHeaIn Song-
dc.contributor.authorVarshney, Gaurav K.-
dc.contributor.authorKim, Yeongjae-
dc.contributor.authorHyemin Song-
dc.contributor.authorSangeun Jeon-
dc.contributor.authorRobbins, Gabrielle-
dc.contributor.authorKim, Sangin-
dc.contributor.authorSuhyeon Yoon-
dc.contributor.authorYong Jun Choi-
dc.contributor.authorKim, Yoo Jung-
dc.contributor.authorBurgess, Shawn-
dc.contributor.authorSukhyun Kang-
dc.contributor.authorSood, Raman-
dc.contributor.authorYoonsung Lee-
dc.contributor.authorKyungjae Myung-
dc.date.accessioned2021-10-15T06:50:09Z-
dc.date.available2021-10-15T06:50:09Z-
dc.date.created2021-08-26-
dc.date.issued2021-11-
dc.identifier.issn1568-7864-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/10415-
dc.description.abstract© 2021A systematic knowledge of the roles of DNA repair genes at the level of the organism has been limited due to the lack of appropriate experimental approaches using animal model systems. Zebrafish has become a powerful vertebrate genetic model system with availability due to the ease of genome editing and large-scale phenotype screening. Here, we generated zebrafish mutants for 32 DNA repair and replication genes through multiplexed CRISPR/Cas9-mediated mutagenesis. Large-scale phenotypic characterization of our mutant collection revealed that three genes (atad5a, ddb1, pcna) are essential for proper embryonic development and hematopoiesis; seven genes (apex1, atrip, ino80, mre11a, shfm1, telo2, wrn) are required for growth and development during juvenile stage and six genes (blm, brca2, fanci, rad51, rad54l, rtel1) play critical roles in sex development. Furthermore, mutation in six genes (atad5a, brca2, polk, rad51, shfm1, xrcc1) displayed hypersensitivity to DNA damage agents. Our zebrafish mutant collection provides a unique resource for understanding of the roles of DNA repair genes at the organismal level.-
dc.language영어-
dc.publisherElsevier B.V.-
dc.titleLarge-scale generation and phenotypic characterization of zebrafish CRISPR mutants of DNA repair genes-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000702850200008-
dc.identifier.scopusid2-s2.0-85112415139-
dc.identifier.rimsid76240-
dc.contributor.affiliatedAuthorKhriezhanuo Nakhro-
dc.contributor.affiliatedAuthorChang-Kyu Oh-
dc.contributor.affiliatedAuthorHeaIn Song-
dc.contributor.affiliatedAuthorHyemin Song-
dc.contributor.affiliatedAuthorSangeun Jeon-
dc.contributor.affiliatedAuthorSuhyeon Yoon-
dc.contributor.affiliatedAuthorYong Jun Choi-
dc.contributor.affiliatedAuthorSukhyun Kang-
dc.contributor.affiliatedAuthorYoonsung Lee-
dc.contributor.affiliatedAuthorKyungjae Myung-
dc.identifier.doi10.1016/j.dnarep.2021.103173-
dc.identifier.bibliographicCitationDNA Repair, v.107-
dc.relation.isPartOfDNA Repair-
dc.citation.titleDNA Repair-
dc.citation.volume107-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaGenetics & Heredity-
dc.relation.journalResearchAreaToxicology-
dc.relation.journalWebOfScienceCategoryGenetics & Heredity-
dc.relation.journalWebOfScienceCategoryToxicology-
dc.subject.keywordPlusEMBRYONIC-DEVELOPMENT-
dc.subject.keywordPlusBLOOMS-SYNDROME-
dc.subject.keywordPlusIN-VITRO-
dc.subject.keywordPlusDAMAGE-
dc.subject.keywordPlusPROTEIN-
dc.subject.keywordPlusMICE-
dc.subject.keywordPlusATM-
dc.subject.keywordPlusMUTATIONS-
dc.subject.keywordPlusMETHYL-
dc.subject.keywordPlusBRCA2-
dc.subject.keywordAuthorCRIPSR/Cas9-
dc.subject.keywordAuthorDNA damage sensitivity-
dc.subject.keywordAuthorDNA repair-
dc.subject.keywordAuthorHematopoiesis-
dc.subject.keywordAuthorMultiplex mutagenesis-
dc.subject.keywordAuthorSex development-
dc.subject.keywordAuthorZebrafish-
Appears in Collections:
Center for Genomic Integrity(유전체 항상성 연구단) > 1. Journal Papers (저널논문)
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