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Fine-tuning of dual-SMAD inhibition to differentiate human pluripotent stem cells into neural crest stem cells

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Title
Fine-tuning of dual-SMAD inhibition to differentiate human pluripotent stem cells into neural crest stem cells
Author(s)
Kim, Hyun-Mun; Noh, Hye Bin; Lee, Sang-Hyuk; Lee, Kun-Gu; Bomi Chang; Cheong, Eunji; 이창준; Hwang, Dong-Youn
Publication Date
2021-09
Journal
CELL PROLIFERATION, v.54, no.9
Publisher
John Wiley and Sons Inc
Abstract
© 2021 The Authors. Cell Proliferation published by John Wiley & Sons Ltd.Objectives: The derivation of neural crest stem cells (NCSCs) from human pluripotent stem cells (hPSCs) has been commonly induced by WNT activation in combination with dual-SMAD inhibition. In this study, by fine-tuning BMP signalling in the conventional dual-SMAD inhibition, we sought to generate large numbers of NCSCs without WNT activation. Materials and methods: In the absence of WNT activation, we modulated the level of BMP signalling in the dual-SMAD inhibition system to identify conditions that efficiently drove the differentiation of hPSCs into NCSCs. We isolated two NCSC populations separately and characterized them in terms of global gene expression profiles and differentiation ability. Results: Our modified dual-SMAD inhibition containing a lower dose of BMP inhibitor than that of the conventional dual-SMAD inhibition drove hPSCs into mainly NCSCs, which consisted of HNK+p75high and HNK+p75low cell populations. We showed that the p75high population formed spherical cell clumps, while the p75low cell population generated a 2D monolayer. We detected substantial differences in gene expression profiles between the two cell groups and showed that both p75high and p75low cells differentiated into mesenchymal stem cells (MSCs), while only p75high cells had the ability to become peripheral neurons. Conclusions: This study will provide a framework for the generation and isolation of NCSC populations for effective cell therapy for peripheral neuropathies and MSC-based cell therapy.
URI
https://pr.ibs.re.kr/handle/8788114/10400
DOI
10.1111/cpr.13103
ISSN
0960-7722
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > 1. Journal Papers (저널논문)
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