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Phosphokinase Antibody Arrays on Dendron-Coated Surface

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Title
Phosphokinase Antibody Arrays on Dendron-Coated Surface
Author(s)
Ju-won Kwak; Hyobin Jeong; Sun-Ho Han; Youngkyu Kim; Sung Min Son; Inhee Mook-Jung; Daehee Hwang; Joon Won Park
Publication Date
2014-05
Journal
PLOS ONE, v.9, no.5, pp.e96456-1 - e96456-9
Publisher
PUBLIC LIBRARY SCIENCE
Abstract
Monitoring protein phosphorylation at the cellular level is important to understand the intracellular signaling. Among the phosphoproteomics methods, phosphokinase antibody arrays have emerged as preferred tools to measure wellcharacterized phosphorylation in the intracellular signaling. Here, we present a dendron-coated phosphokinase antibody array (DPA) in which the antibodies are immobilized on a dendron-coated glass slide. Self-assembly of conically shaped dendrons well-controlled in size and structure resulted in precisely controlled lateral spacing between the immobilized phosphosite-specific antibodies, leading to minimized steric hindrance and improved antigen-antibody binding kinetics. These features increased sensitivity, selectivity, and reproducibility in measured amounts of protein phosphorylation. To demonstrate the utility of the DPA, we generated the phosphorylation profiles of brain tissue samples obtained from Alzheimer’s disease (AD) model mice. The analysis of the profiles revealed signaling pathways deregulated during the course of AD progression.
URI
http://pr.ibs.re.kr/handle/8788114/1025
DOI
10.1371/journal.pone.0096456
ISSN
1932-6203
Appears in Collections:
Center for Plant Aging Research (식물 노화·수명 연구단) > Journal Papers (저널논문)
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