BROWSE

Related Scientist

hwang,daehee's photo.

hwang,daehee
식물노화·수명연구단
more info

ITEM VIEW & DOWNLOAD

Phosphokinase Antibody Arrays on Dendron-Coated Surface

DC Field Value Language
dc.contributor.authorJu-won Kwak-
dc.contributor.authorHyobin Jeong-
dc.contributor.authorSun-Ho Han-
dc.contributor.authorYoungkyu Kim-
dc.contributor.authorSung Min Son-
dc.contributor.authorInhee Mook-Jung-
dc.contributor.authorDaehee Hwang-
dc.contributor.authorJoon Won Park-
dc.date.available2015-04-20T05:54:38Z-
dc.date.created2014-11-24ko
dc.date.issued2014-05-
dc.identifier.issn1932-6203-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/1025-
dc.description.abstractMonitoring protein phosphorylation at the cellular level is important to understand the intracellular signaling. Among the phosphoproteomics methods, phosphokinase antibody arrays have emerged as preferred tools to measure wellcharacterized phosphorylation in the intracellular signaling. Here, we present a dendron-coated phosphokinase antibody array (DPA) in which the antibodies are immobilized on a dendron-coated glass slide. Self-assembly of conically shaped dendrons well-controlled in size and structure resulted in precisely controlled lateral spacing between the immobilized phosphosite-specific antibodies, leading to minimized steric hindrance and improved antigen-antibody binding kinetics. These features increased sensitivity, selectivity, and reproducibility in measured amounts of protein phosphorylation. To demonstrate the utility of the DPA, we generated the phosphorylation profiles of brain tissue samples obtained from Alzheimer’s disease (AD) model mice. The analysis of the profiles revealed signaling pathways deregulated during the course of AD progression.-
dc.description.uri1-
dc.language영어-
dc.publisherPUBLIC LIBRARY SCIENCE-
dc.titlePhosphokinase Antibody Arrays on Dendron-Coated Surface-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000338029800070-
dc.identifier.scopusid2-s2.0-84900426234-
dc.identifier.rimsid16538ko
dc.date.tcdate2018-10-01-
dc.contributor.affiliatedAuthorDaehee Hwang-
dc.identifier.doi10.1371/journal.pone.0096456-
dc.identifier.bibliographicCitationPLOS ONE, v.9, no.5, pp.e96456-1 - e96456-9-
dc.citation.titlePLOS ONE-
dc.citation.volume9-
dc.citation.number5-
dc.citation.startPagee96456-1-
dc.citation.endPagee96456-9-
dc.date.scptcdate2018-10-01-
dc.description.wostc3-
dc.description.scptc3-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
Appears in Collections:
Center for Plant Aging Research (식물 노화·수명 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
Phosphokinase antibody arrays on Dendron-coated surface.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse