BROWSE

Related Scientist

cinap's photo.

cinap
나노구조물리연구단
more info

ITEM VIEW & DOWNLOAD

Simple method of DNA stretching on glass substrate for fluorescence imaging and spectroscopy

DC Field Value Language
dc.contributor.authorGuru P. Neupane-
dc.contributor.authorKrishna P. Dhakal-
dc.contributor.authorMin Su Kim-
dc.contributor.authorLee H.-
dc.contributor.authorGuthold M.-
dc.contributor.authorJoseph V.S.-
dc.contributor.authorHong J.-D.-
dc.contributor.authorJeongyong Kim-
dc.date.available2015-04-20T05:53:49Z-
dc.date.created2014-08-11ko
dc.date.issued2014-05-
dc.identifier.issn1083-3668-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/1022-
dc.description.abstractWe demonstrate a simple method of stretching DNA to its full length, suitable for optical imaging and atomic force microscopy (AFM). Two competing forces on the DNA molecules, which are the electrostatic attraction between positively charged dye molecules (YOYO-1) intercalated into DNA and the negatively charged surface of glass substrate, and the centrifugal force of the rotating substrate, are mainly responsible for the effective stretching and the dispersion of single strands of DNA. The density of stretched DNA molecules could be controlled by the concentration of the dye-stained DNA solution. Stretching of single DNA molecules was confirmed by AFM imaging and the photoluminescence spectra of single DNA molecule stained with YOYO-1 were obtained, suggesting that our method is useful for spectroscopic analysis of DNA at the single molecule level. © 2014 Society of Photo-Optical Instrumentation Engineers.-
dc.description.uri1-
dc.language영어-
dc.publisherSPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS-
dc.subjectDNA-
dc.subjectstretching-
dc.subjectsurface treatment-
dc.subjectatomic force microscopy-
dc.subjectphotoluminescence.-
dc.titleSimple method of DNA stretching on glass substrate for fluorescence imaging and spectroscopy-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000338334600011-
dc.identifier.scopusid2-s2.0-84892145830-
dc.identifier.rimsid414ko
dc.date.tcdate2018-10-01-
dc.contributor.affiliatedAuthorGuru P. Neupane-
dc.contributor.affiliatedAuthorKrishna P. Dhakal-
dc.contributor.affiliatedAuthorMin Su Kim-
dc.contributor.affiliatedAuthorJeongyong Kim-
dc.identifier.doi10.1117/1.JBO.19.5.051210-
dc.identifier.bibliographicCitationJOURNAL OF BIOMEDICAL OPTICS, v.19, no.5, pp.51210-
dc.citation.titleJOURNAL OF BIOMEDICAL OPTICS-
dc.citation.volume19-
dc.citation.number5-
dc.citation.startPage51210-
dc.date.scptcdate2018-10-01-
dc.description.wostc7-
dc.description.scptc7-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.subject.keywordAuthoratomic force microscopy-
dc.subject.keywordAuthorphotoluminescence-
dc.subject.keywordAuthorstretching-
dc.subject.keywordAuthorsurface treatment-
Appears in Collections:
Center for Integrated Nanostructure Physics(나노구조물리 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
333. JBO_19_5_051210.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse