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Lee, Seong Sin
식물 노화·수명 연구단
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Protocol for whole-brain immunostaining of the turquoise killifish after tissue clearing

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Title
Protocol for whole-brain immunostaining of the turquoise killifish after tissue clearing
Author(s)
Seongsin Lee; Yumi Kim
Publication Date
2021-06
Journal
STAR Protocols, v.2, no.2
Publisher
Cell Press
Abstract
© 2021 The AuthorsDistribution of cells and proteins in whole organs, such as the brain, can provide another level of insight into molecular and cellular functions. Here, we describe a whole-brain immunostaining method using the turquoise killifish, an emerging model for aging research. We optimized a protocol for tissue clearing and whole-brain immunostaining to the turquoise killifish brain. This protocol provides a comprehensive procedure from brain dissection to whole-brain imaging and image processing. For complete details on the use and execution of this protocol, please refer to Eunjeong Do (2020) and Lee et al. (2021).
URI
https://pr.ibs.re.kr/handle/8788114/10083
DOI
10.1016/j.xpro.2021.100564
ISSN
2666-1667
Appears in Collections:
Center for Plant Aging Research (식물 노화·수명 연구단) > 1. Journal Papers (저널논문)
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