BROWSE

Related Scientist

Koo, Heejung's photo.

Koo, Heejung
식물 노화·수명 연구단
more info

ITEM VIEW & DOWNLOAD

Transfer RNA-derived fragments in aging Caenorhabditis elegans originate from abundant homologous gene copies

Cited 0 time in webofscience Cited 0 time in scopus
20 Viewed 0 Downloaded
Title
Transfer RNA-derived fragments in aging Caenorhabditis elegans originate from abundant homologous gene copies
Author(s)
Shin, GiWon; Hee Jung Koo; Seo, Mihwa; Lee, Seung-Jae V.; HongGil Nam; Jung, Gyoo Yeol
Publication Date
2021-06
Journal
SCIENTIFIC REPORTS, v.11, no.1
Publisher
Nature Research
Abstract
© 2021, The Author(s).Small RNAs that originate from transfer RNA (tRNA) species, tRNA-derived fragments (tRFs), play diverse biological functions but little is known for their association with aging. Moreover, biochemical aspects of tRNAs limit discovery of functional tRFs by high throughput sequencing. In particular, genes encoding tRNAs exist as multiple copies throughout genome, and mature tRNAs have various modified bases, contributing to ambiguities for RNA sequencing-based analysis of tRFs. Here, we report age-dependent changes of tRFs in Caenorhabditis elegans. We first analyzed published RNA sequencing data by using a new strategy for tRNA-associated sequencing reads. Our current method used unique mature tRNAs as a reference for the sequence alignment, and properly filtered out false positive enrichment for tRFs. Our analysis successfully distinguished de novo mutation sites from differences among homologous copies, and identified potential RNA modification sites. Overall, the majority of tRFs were upregulated during aging and originated from 5′-ends, which we validated by using Northern blot analysis. Importantly, we revealed that the major source of tRFs upregulated during aging was the tRNAs with abundant gene copy numbers. Our analysis suggests that tRFs are useful biomarkers of aging particularly when they originate from abundant homologous gene copies.
URI
https://pr.ibs.re.kr/handle/8788114/10075
DOI
10.1038/s41598-021-91724-z
ISSN
2045-2322
Appears in Collections:
Center for Plant Aging Research (식물 노화·수명 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
There are no files associated with this item.

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse