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Koo, Heejung
식물 노화·수명 연구단
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Transfer RNA-derived fragments in aging Caenorhabditis elegans originate from abundant homologous gene copies

DC Field Value Language
dc.contributor.authorShin, GiWon-
dc.contributor.authorHee Jung Koo-
dc.contributor.authorSeo, Mihwa-
dc.contributor.authorLee, Seung-Jae V.-
dc.contributor.authorHongGil Nam-
dc.contributor.authorJung, Gyoo Yeol-
dc.date.accessioned2021-08-11T05:50:12Z-
dc.date.accessioned2021-08-11T05:50:12Z-
dc.date.available2021-08-11T05:50:12Z-
dc.date.available2021-08-11T05:50:12Z-
dc.date.created2021-07-07-
dc.date.issued2021-06-10-
dc.identifier.issn2045-2322-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/10075-
dc.description.abstract© 2021, The Author(s).Small RNAs that originate from transfer RNA (tRNA) species, tRNA-derived fragments (tRFs), play diverse biological functions but little is known for their association with aging. Moreover, biochemical aspects of tRNAs limit discovery of functional tRFs by high throughput sequencing. In particular, genes encoding tRNAs exist as multiple copies throughout genome, and mature tRNAs have various modified bases, contributing to ambiguities for RNA sequencing-based analysis of tRFs. Here, we report age-dependent changes of tRFs in Caenorhabditis elegans. We first analyzed published RNA sequencing data by using a new strategy for tRNA-associated sequencing reads. Our current method used unique mature tRNAs as a reference for the sequence alignment, and properly filtered out false positive enrichment for tRFs. Our analysis successfully distinguished de novo mutation sites from differences among homologous copies, and identified potential RNA modification sites. Overall, the majority of tRFs were upregulated during aging and originated from 5′-ends, which we validated by using Northern blot analysis. Importantly, we revealed that the major source of tRFs upregulated during aging was the tRNAs with abundant gene copy numbers. Our analysis suggests that tRFs are useful biomarkers of aging particularly when they originate from abundant homologous gene copies.-
dc.language영어-
dc.publisherNature Research-
dc.titleTransfer RNA-derived fragments in aging Caenorhabditis elegans originate from abundant homologous gene copies-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000663785600054-
dc.identifier.scopusid2-s2.0-85107462244-
dc.identifier.rimsid75965-
dc.contributor.affiliatedAuthorHee Jung Koo-
dc.contributor.affiliatedAuthorHongGil Nam-
dc.identifier.doi10.1038/s41598-021-91724-z-
dc.identifier.bibliographicCitationSCIENTIFIC REPORTS, v.11, no.1-
dc.relation.isPartOfSCIENTIFIC REPORTS-
dc.citation.titleSCIENTIFIC REPORTS-
dc.citation.volume11-
dc.citation.number1-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaScience & Technology - Other Topics-
dc.relation.journalWebOfScienceCategoryMultidisciplinary Sciences-
dc.subject.keywordPlusIDENTIFICATION-
dc.subject.keywordPlusMICRORNA-
dc.subject.keywordPlusCLEAVAGE-
dc.subject.keywordPlusSTRESS-
dc.subject.keywordPlusTRFS-
dc.subject.keywordPlusSEQ-
dc.subject.keywordPlusREVERSE TRANSCRIPTION-
dc.subject.keywordPlusTRANSLATION FIDELITY-
dc.subject.keywordPlusSEQUENCING REVEALS-
Appears in Collections:
Center for Plant Aging Research (식물 노화·수명 연구단) > 1. Journal Papers (저널논문)
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