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The SARS-CoV-2 RNA interactome

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Title
The SARS-CoV-2 RNA interactome
Author(s)
Sungyul Lee; Young-suk Lee; Yeon Choi; Ahyeon Son; Youngran Park; Kyung-Min Lee; Jeesoo Kim; Jong-Seo Kim; V. Narry Kim
Subject
GENE-EXPRESSION, ; PROTEIN, ; CORONAVIRUS, ; TRANSLATION, ; BINDING, ; INTERFERON, ; NSP9, ; IDENTIFICATION, ; REPLICATION, ; MODULATION
Publication Date
2021-07-01
Journal
MOLECULAR CELL, v.81, no.13, pp.2838 - 2850
Publisher
Cell Press
Abstract
© 2021 Elsevier Inc.SARS-CoV-2 is an RNA virus whose success as a pathogen relies on its abilities to repurpose host RNA-binding proteins (RBPs) and to evade antiviral RBPs. To uncover the SARS-CoV-2 RNA interactome, we here develop a robust ribonucleoprotein (RNP) capture protocol and identify 109 host factors that directly bind to SARS-CoV-2 RNAs. Applying RNP capture on another coronavirus, HCoV-OC43, revealed evolutionarily conserved interactions between coronaviral RNAs and host proteins. Transcriptome analyses and knockdown experiments delineated 17 antiviral RBPs, including ZC3HAV1, TRIM25, PARP12, and SHFL, and 8 proviral RBPs, such as EIF3D and CSDE1, which are responsible for co-opting multiple steps of the mRNA life cycle. This also led to the identification of LARP1, a downstream target of the mTOR signaling pathway, as an antiviral host factor that interacts with the SARS-CoV-2 RNAs. Overall, this study provides a comprehensive list of RBPs regulating coronaviral replication and opens new avenues for therapeutic interventions.
URI
https://pr.ibs.re.kr/handle/8788114/9860
DOI
10.1016/j.molcel.2021.04.022
ISSN
1097-2765
Appears in Collections:
Center for RNA Research(RNA 연구단) > 1. Journal Papers (저널논문)
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