Enrichment of cells with TALEN-induced mutations using surrogate reporters

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Title
Enrichment of cells with TALEN-induced mutations using surrogate reporters
Author(s)
Young-Hoon Kim; Suresh Ramakrishna; Hyongbum Kim; Jin-Soo Kim
Publication Date
2014-08
Journal
METHODS, v.69, no.1, pp.108 - 117
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Abstract
Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always high enough to allow the efficient derivation of cells containing TALEN-driven mutations. Thus, simple methods to enrich cells containing TALEN-directed mutations would facilitate the use of TALENs. Here we describe the enrichment of such cells using surrogate episomal reporters coupled with flow cytometric sorting, magnetic separation, or hygromycin selection.
URI
https://pr.ibs.re.kr/handle/8788114/962
ISSN
1046-2023
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > Journal Papers (저널논문)
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