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나노의학 연구단
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Integrated Dual-Mode Chromatography to Enrich Extracellular Vesicles from Plasma

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dc.contributor.authorJan Van Deun-
dc.contributor.authorAla Jo-
dc.contributor.authorHuiyan Li-
dc.contributor.authorHsing-Ying Lin-
dc.contributor.authorRalph Weissleder-
dc.contributor.authorHyungsoon Im-
dc.contributor.authorHakho Lee-
dc.date.accessioned2021-01-08T01:50:00Z-
dc.date.accessioned2021-01-08T01:50:00Z-
dc.date.available2021-01-08T01:50:00Z-
dc.date.available2021-01-08T01:50:00Z-
dc.date.created2020-06-12-
dc.date.issued2020-12-
dc.identifier.issn2366-7478-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/9013-
dc.description.abstractPurifying extracellular vesicles (EVs) from complex biological fluids is a critical step in analyzing EVs molecularly. Plasma lipoprotein particles (LPPs) are a significant confounding factor as they outnumber EVs >104-fold. Given their overlap in size, LPPs cannot be completely removed using standard size-exclusion chromatography. Density-based separation of LPPs can be applied but is impractical for routine use in clinical research and practice. Here a new separation approach, known as dual-mode chromatography (DMC), capable of enriching plasma EVs, and depleting LPPs is reported. DMC conveniently integrates two orthogonal separation steps in a single column device: i) size exclusion to remove high-density lipoproteins (HDLs) that are smaller than EVs; and ii) cation exchange to clear positively charged ApoB100-containing LPPs, mostly (very) low-density lipoproteins (V)LDLs, from negatively charged EVs. The strategy enables DMC to deplete most LPPs (>97% of HDLs and >99% of (V)LDLs) from human plasma, while retaining EVs (>30% of input). Furthermore, the two-in-one operation is fast (15 min per sample) and equipment-free. With abundant LPPs removed, DMC-prepared samples facilitate EV identification in imaging analyses and improve the accuracy for EV protein analysis-
dc.description.uri1-
dc.language영어-
dc.publisherWILEY-V C H VERLAG GMB-
dc.titleIntegrated Dual-Mode Chromatography to Enrich Extracellular Vesicles from Plasma-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000529672100001-
dc.identifier.scopusid2-s2.0-85083999358-
dc.identifier.rimsid72029-
dc.contributor.affiliatedAuthorHakho Lee-
dc.identifier.doi10.1002/adbi.201900310-
dc.identifier.bibliographicCitationAdvanced Biosystems, v.4, no.12-
dc.citation.titleAdvanced Biosystems-
dc.citation.volume4-
dc.citation.number12-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.subject.keywordAuthorlipoproteins-
dc.subject.keywordAuthorliquid biopsies-
dc.subject.keywordAuthorextracellular vesicles-
Appears in Collections:
Center for Nanomedicine (나노의학 연구단) > 1. Journal Papers (저널논문)
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