BROWSE

Related Scientist

's photo.

인지 및 사회성 연구단
more info

ITEM VIEW & DOWNLOAD

Platycodin D enhances LDLR expression and LDL uptake via down-regulation of IDOL mRNA in hepatic cells

Cited 0 time in webofscience Cited 0 time in scopus
100 Viewed 0 Downloaded
Title
Platycodin D enhances LDLR expression and LDL uptake via down-regulation of IDOL mRNA in hepatic cells
Author(s)
Choi, Y.-J.; Sol Ji Lee; Kim, H.I.; Lee, H.J.; Kang, S.J.; TaiYoung Kim; Cheon, C.; Ko, S.-G.
Publication Date
2020-11
Journal
SCIENTIFIC REPORTS, v.10, no.1, pp.19834
Publisher
NATURE PUBLISHING GROUP
Abstract
© 2020, The Author(s).The root of Platycodon grandiflorum (PG) has long been used as a traditional herbal medicine in Asian country. Platycondin D (PD), triterpenoid saponin that is a main constituent of PG, exhibits various biological activities such as anti-inflammatory, anti-oxidant, anti-diabetic, and anti-cancer effects. A previous study showed that PD had cholesterol-lowering effects in mice that develop hypercholesterolemia, but the underlying molecular mechanisms have not been elucidated during the last decade. Here, we demonstrated that both PG and PD markedly increased levels of cell surface low-density lipoprotein receptor (LDLR) by down-regulation of the E3 ubiquitin ligase named inducible degrader of the LDLR (IDOL) mRNA, leading to the enhanced uptake of LDL-derived cholesterol (LDL-C) in hepatic cells. Furthermore, cycloheximide chase analysis and in vivo ubiquitination assay revealed that PD increased the half-life of LDLR protein by reducing IDOL-mediated LDLR ubiquitination. Finally, we demonstrated that treatment of HepG2 cells with simvastatin in combination with PG and PD had synergistic effects on the improvement of LDLR expression and LDL-C uptake. Together, these results provide the first molecular evidence for anti-hypercholesterolemic activity of PD and suggest that PD alone or together with statin could be a potential therapeutic option in the treatment of atherosclerotic cardiovascular disease
URI
https://pr.ibs.re.kr/handle/8788114/8923
DOI
10.1038/s41598-020-76224-w
ISSN
2045-2322
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
There are no files associated with this item.

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse