BROWSE

Related Scientist

hwang,daehee's photo.

hwang,daehee
식물노화·수명연구단
more info

ITEM VIEW & DOWNLOAD

Urinary Proteome Profile Predictive of Disease Activity in Rheumatoid Arthritis

Cited 16 time in webofscience Cited 13 time in scopus
1,487 Viewed 289 Downloaded
Title
Urinary Proteome Profile Predictive of Disease Activity in Rheumatoid Arthritis
Author(s)
Kang, MJ; Park, YJ; You, S; Yoo, SA; Choi, S; Kim, DH; Cho, CS; Yi, EC; Daehee Hwang; Kim, WU
Subject
rheumatoid arthritis, urine, biomarkers, soluble CD14, LC−MS/MS analysis
Publication Date
2014-11
Journal
JOURNAL OF PROTEOME RESEARCH, v.13, no.11, pp.5206 - 5217
Publisher
AMER CHEMICAL SOC
Abstract
Current serum biomarkers for rheumatoid arthritis (RA) are not highly sensitive or specific to changes of disease activities. Thus, other complementary biomarkers have been needed to improve assessment of RA activities. In many diseases, urine has been studied as a window to provide complementary information to serum measures. Here, we conducted quantitative urinary proteome profiling using liquid chromatography−tandem mass spectrometry (LC−MS/MS) and identified 134 differentially expressed proteins (DEPs) between RA and osteoarthritis (OA) urine samples. By integrating the DEPs with gene expression profiles in joints and mononuclear cells, we initially selected 12 biomarker candidates related to joint pathology and then tested their altered expression in independent RA and OA samples using enzyme-linked immunosorbent assay. Of the initial candidates, we selected four DEPs as final candidates that were abundant in RA patients and consistent with those observed in LC−MS/MS analysis. Among them, we further focused on urinary soluble CD14 (sCD14) and examined its diagnostic value and association with disease activity. Urinary sCD14 had a diagnostic value comparable to conventional serum measures and an even higher predictive power for disease activity when combined with serum C-reactive protein. Thus, our urinary proteome provides a diagnostic window complementary to current serum parameters for the disease activity of RA.
URI
https://pr.ibs.re.kr/handle/8788114/870
DOI
10.1021/pr500467d
ISSN
1535-3893
Appears in Collections:
Center for Plant Aging Research (식물 노화·수명 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
15. Urinary proteome profile.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse