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Uridylation by TUT4 and TUT7 marks mRNA for degradation

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Title
Uridylation by TUT4 and TUT7 marks mRNA for degradation
Author(s)
Jaechul Lim; Minju Ha; Hyeshik Chang; S. Chul Kwon; Dhirendra K. Simanshu; Dinshaw J. Patel; V. Narry Kim
Publication Date
2014-12
Journal
CELL, v.159, no.6, pp.1365 - 1376
Publisher
CELL PRESS
Abstract
Uridylation occurs pervasively on mRNAs, yet its mechanism and significance remain unknown. By applying TAIL-seq, we identify TUT4 and TUT7 (TUT4/7), also known as ZCCHC11 and ZCCHC6, respectively, as mRNA uridylation enzymes. Uridylation readily occurs on deadenylated mRNAs in cells. Consistently, purified TUT4/7 selectively recognize and uridylate RNAs with short A-tails (less than 25 nt) in vitro. PABPC1 antagonizes uridylation of polyadenylated mRNAs, contributing to the specificity for short A-tails. In cells depleted of TUT4/7, the vast majority of mRNAs lose the oligo-U-tails, and their half-lives are extended. Suppression of mRNA decay factors leads to the accumulation of oligo-uridylated mRNAs. In line with this, microRNA induces uridylation of its targets, and TUT4/7 are required for enhanced decay of microRNA targets. Our study explains the mechanism underlying selective uridylation of deadenylated mRNAs and demonstrates a fundamental role of oligo-U-tail as a molecular mark for global mRNA decay.
URI
https://pr.ibs.re.kr/handle/8788114/839
ISSN
0092-8674
Appears in Collections:
Center for RNA Research(RNA 연구단) > Journal Papers (저널논문)
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