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Kim, Ho Min
단백질 커뮤니케이션 그룹
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Neural circuit analysis using a novel intersectional split intein-mediated split-Cre recombinase system

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Title
Neural circuit analysis using a novel intersectional split intein-mediated split-Cre recombinase system
Author(s)
Audrey Tze Ting Khoo; Paul Jong Kim; Ho Min Kim; H. Shawn Je
Subject
Circuit mapping, ; DLX6, ; GABA, ; Projection neurons, ; Retrograde virus, ; Split intein, ; Split-cre recombinase, ; Transgenic mouse
Publication Date
2020-07
Journal
Molecular Brain, v.13, no.1, pp.101
Publisher
BIOMED CENTRAL LTD
Abstract
© The Author(s). The defining features of a neuron are its functional and anatomical connections with thousands of other neurons in the brain. Together, these neurons form functional networks that direct animal behavior. Current approaches that allow the interrogation of specific populations of neurons and neural circuits rely heavily on targeting their gene expression profiles or connectivity. However, these approaches are often unable to delineate specific neuronal populations. Here, we developed a novel intersectional split intein-mediated split-Cre recombinase system that can selectively label specific types of neurons based on their gene expression profiles and structural connectivity. We developed this system by splitting Cre recombinase into two fragments with evolved split inteins and subsequently expressed one fragment under the influence of a cell type-specific promoter in a transgenic animal, and delivered the other fragment via retrograde viral gene transfer. This approach results in the reconstitution of Cre recombinase in only specific population of neurons projecting from a specific brain region or in those of a specific neuronal type. Taken together, our split intein-based split-Cre system will be useful for sophisticated characterization of mammalian brain circuits
URI
https://pr.ibs.re.kr/handle/8788114/7781
DOI
10.1186/s13041-020-00640-2
ISSN
1756-6606
Appears in Collections:
Pioneer Research Center for Biomolecular and Cellular Structure(바이오분자 및 세포구조 연구단) > Protein Communication Group(단백질 커뮤니케이션 그룹) > 1. Journal Papers (저널논문)
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