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mRNA destabilization is the dominant effect of mammalian microRNAs by the time substantial repression ensues

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Title
mRNA destabilization is the dominant effect of mammalian microRNAs by the time substantial repression ensues
Author(s)
Eichhorn SW; Guo H; McGeary SE; Rodriguez-Mias RA; Shin C; Daehyun Baek; Hsu SH; Ghoshal K; Villén J; Bartel DP
Publication Date
2014-10
Journal
MOLECULAR CELL, v.56, no.1, pp.104 - 115
Publisher
CELL PRESS
Abstract
MicroRNAs (miRNAs) regulate target mRNAs through a combination of translational repression and mRNA destabilization, with mRNA destabilization dominating at steady state in the few contexts examined globally. Here, we extend the global steady-state measurements to additional mammalian contexts and find that regardless of the miRNA, cell type, growth condition, or translational state, mRNA destabilization explains most (66%.>90%) miRNAmediated repression. We also determine the relative dynamics of translational repression and mRNA destabilization for endogenous mRNAs as a miRNA is induced. Although translational repression occurs rapidly, its effect is relatively weak, such that by the time consequential repression ensues, the effect of mRNA destabilization dominates. These results imply that consequential miRNA-mediated repression is largely irreversible and provide other insights into the nature of miRNA-mediated regulation. They also simplify future studies, dramatically extending the known contexts and time points for which monitoring mRNA changes captures most of the direct miRNA effects.
URI
https://pr.ibs.re.kr/handle/8788114/769
ISSN
1097-2765
Appears in Collections:
Center for RNA Research(RNA 연구단) > Journal Papers (저널논문)
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