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Human ANKLE1 Is a Nuclease Specific for Branched DNA

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Title
Human ANKLE1 Is a Nuclease Specific for Branched DNA
Author(s)
Junfang Song; Alasdair D.J. Freeman; Axel Knebel; Anton Gartner; David M.J. Lilley
Subject
chromosome segregation, ; helical junction resolution, ; LEM-3, ; ultrafine DNA bridges
Publication Date
2020-10
Journal
JOURNAL OF MOLECULAR BIOLOGY, v.432, no.21, pp.5825 - 5834
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Abstract
© 2020 The Authors. All physical connections between sister chromatids must be broken before cells can divide, and eukaryotic cells have evolved multiple ways in which to process branchpoints connecting DNA molecules separated both spatially and temporally. A single DNA link between chromatids has the potential to disrupt cell cycle progression and genome integrity, so it is highly likely that cells require a nuclease that can process remaining unresolved and hemi-resolved DNA junctions and other branched species at the very late stages of mitosis. We argue that ANKLE1 probably serves this function in human cells (LEM-3 in Caenorhabditis elegans). LEM-3 has previously been shown to be located at the cell mid-body, and we show here that human ANKLE1 is a nuclease that cleaves a range of branched DNA species. It thus has the substrate selectivity consistent with an enzyme required to process a variety of unresolved and hemi-resolved branchpoints in DNA. Our results suggest that ANKLE1 acts as a catch-all enzyme of last resort that allows faithful chromosome segregation and cell division to occur
URI
https://pr.ibs.re.kr/handle/8788114/7595
DOI
10.1016/j.jmb.2020.08.022
ISSN
0022-2836
Appears in Collections:
Center for Genomic Integrity(유전체 항상성 연구단) > 1. Journal Papers (저널논문)
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