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Genomic Clustering Facilitates Nuclear Processing of Suboptimal Pri-miRNA Loci

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Title
Genomic Clustering Facilitates Nuclear Processing of Suboptimal Pri-miRNA Loci
Author(s)
Shang R.; S. Chan Baek; Kijun Kim; Boseon Kim; V. Narry Kim; Lai E.C.
Publication Date
2020-04
Journal
MOLECULAR CELL, v.78, no.2, pp.303 - 316.e4
Publisher
CELL PRESS
Abstract
© 2020 Elsevier Inc.Nuclear processing of most miRNAs is mediated by Microprocessor, comprised of RNase III enzyme Drosha and its cofactor DGCR8. Here, we uncover a hidden layer of Microprocessor regulation via studies of Dicer-independent mir-451, which is clustered with canonical mir-144. Although mir-451 is fully dependent on Drosha/DGCR8, its short stem and small terminal loop render it an intrinsically weak Microprocessor substrate. Thus, it must reside within a cluster for normal biogenesis, although the identity and orientation of its neighbor are flexible. We use DGCR8 tethering assays and operon structure-function assays to demonstrate that local recruitment and transfer of Microprocessor enhances suboptimal substrate processing. This principle applies more broadly since genomic analysis indicates suboptimal canonical miRNAs are enriched in operons, and we validate several of these experimentally. Proximity-based enhancement of suboptimal hairpin processing provides a rationale for genomic retention of certain miRNA operons and may explain preferential evolutionary emergence of miRNA operons. Shang et al. investigate the biogenesis of clustered miRNAs, which were largely considered to be processed independently. They document multiple mammalian miRNA operons bearing suboptimal substrates of the Drosha/DGCR8 (Microprocessor) complex, which require close proximity to canonical miRNA neighbors to recruit and locally transfer Microprocessor for their optimal nuclear biogenesis
URI
https://pr.ibs.re.kr/handle/8788114/7130
ISSN
1097-2765
Appears in Collections:
Center for RNA Research(RNA 연구단) > Journal Papers (저널논문)
Files in This Item:
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