BROWSE

Related Scientist

Researcher

RNA 연구단
RNA 연구단
more info

NSUN2 introduces 5-methylcytosines in mammalian mitochondrial tRNAs

Cited 0 time in webofscience Cited 0 time in scopus
9 Viewed 1 Downloaded
Title
NSUN2 introduces 5-methylcytosines in mammalian mitochondrial tRNAs
Author(s)
Van Haute, L; Lee, SY; McCann, BJ; Powell, CA; Bansal, D; Vasiliauskaite, L; Garone, C; Sanghee Shin; Jong-Seo Kim; Frye, M; Gleeson, JG; Miska, EA; Rhee, HW; Minczuk, M
Publication Date
2019-09
Journal
NUCLEIC ACIDS RESEARCH, v.47, no.16, pp.8720 - 8733
Publisher
OXFORD UNIV PRESS
Abstract
© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.Expression of human mitochondrial DNA is indispensable for proper function of the oxidative phosphorylation machinery. The mitochondrial genome encodes 22 tRNAs, 2 rRNAs and 11 mRNAs and their post-transcriptional modification constitutes one of the key regulatory steps during mitochondrial gene expression. Cytosine-5 methylation (m5C) has been detected in mitochondrial transcriptome, however its biogenesis has not been investigated in details. Mammalian NOP2/Sun RNA Methyltransferase Family Member 2 (NSUN2) has been characterized as an RNA methyltransferase introducing m5C in nuclear-encoded tRNAs, mRNAs and microRNAs and associated with cell proliferation and differentiation, with pathogenic variants in NSUN2 being linked to neurodevelopmental disorders. Here we employ spatially restricted proximity labelling and immunodetection to demonstrate that NSUN2 is imported into the matrix of mammalian mitochondria. Using three genetic models for NSUN2 inactivation-knockout mice, patient-derived fibroblasts and CRISPR/Cas9 knockout in human cells-we show that NSUN2 is necessary for the generation of m5C at positions 48, 49 and 50 of several mammalian mitochondrial tRNAs. Finally, we show that inactivation of NSUN2 does not have a profound effect on mitochondrial tRNA stability and oxidative phosphorylation in differentiated cells. We discuss the importance of the newly discovered function of NSUN2 in the context of human disease
URI
https://pr.ibs.re.kr/handle/8788114/6582
ISSN
0305-1048
Appears in Collections:
Center for RNA Research(RNA 연구단) > Journal Papers (저널논문)
Files in This Item:
gkz559.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse