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유전체교정 연구단
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Adenine base editors catalyze cytosine conversions in human cells

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Title
Adenine base editors catalyze cytosine conversions in human cells
Author(s)
Heon Seok Kim; Jeong Y.K.; Hur J.K.; Jin-Soo Kim; Bae S.
Publication Date
2019-10
Journal
NATURE BIOTECHNOLOGY, v.37, no.10, pp.1145 - 1148
Publisher
NATURE PUBLISHING GROUP
Abstract
Adenine base editors comprise an adenosine deaminase, evolved in vitro, and a Cas9 nickase. Here, we show that in addition to converting adenine to guanine, adenine base editors also convert cytosine to guanine or thymine in a narrow editing window (positions 5-7) and in a confined TC*N sequence context. Adenine base editor-induced cytosine substitutions occur independently of adenosine conversions with an efficiency of up to 11.2% and reduce the number of suitable targeting sites for high-specificity base editing. © The Author(s), under exclusive licence to Springer Nature America, Inc. 2019 Nature
URI
https://pr.ibs.re.kr/handle/8788114/6411
ISSN
1087-0156
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > Journal Papers (저널논문)
Files in This Item:
1910_김헌석_Nature Biotechnology.pdfDownload

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