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유전체 교정 연구단
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Evaluating and enhancing target specificity of gene-editing nucleases and deaminases

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Title
Evaluating and enhancing target specificity of gene-editing nucleases and deaminases
Author(s)
Daesik Kim; Luk K.; Wolfe S.A.; Jin-Soo Kim
Publication Date
2019-03
Journal
ANNUAL REVIEW OF BIOCHEMISTRY, v.88, no., pp.191 - 220
Publisher
ANNUAL REVIEWS
Abstract
© 2019 by Annual Reviews. All rights reserved.Programmable nucleases and deaminases, which include zinc-finger nucleases, transcription activator-like effector nucleases, CRISPR RNA-guided nucleases, and RNA-guided base editors, are now widely employed for the targeted modification of genomes in cells and organisms. These gene-editing tools hold tremendous promise for therapeutic applications. Importantly, these nucleases and deaminases may display off-target activity through the recognition of near-cognate DNA sequences to their target sites, resulting in collateral damage to the genome in the form of local mutagenesis or genomic rearrangements. For therapeutic genome-editing applications with these classes of programmable enzymes, it is essential to measure and limit genome-wide off-target activity. Herein, we discuss the key determinants of off-target activity for these systems. We describe various cell-based and cell-free methods for identifying genome-wide off-target sites and diverse strategies that have been developed for reducing the off-target activity of programmable gene-editing enzymes
URI
https://pr.ibs.re.kr/handle/8788114/6381
ISSN
0066-4154
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > Journal Papers (저널논문)
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1903_김대식_ANNUAL REVIEW OF BIOCHEMISTRY_evaluating and enhancing.pdfDownload

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