BROWSE

Related Scientist

kim,hyunsuk's photo.

kim,hyunsuk
유전체항상성연구단
more info

ITEM VIEW & DOWNLOAD

MiR223-3p promotes synthetic lethality in BRCA1-deficient cancers

Cited 6 time in webofscience Cited 6 time in scopus
836 Viewed 191 Downloaded
Title
MiR223-3p promotes synthetic lethality in BRCA1-deficient cancers
Author(s)
Srinivasan G.; Williamson E.A.; Kong K.; Jaiswal A.S.; Huang G.; Hyun-Suk Kim; Orlando Schärer; Zhao W.; Burma S.; Patrick Sung; Hromas R.
Subject
DNA repair, ; MicroRNA, ; Oncogenesis, ; Replication fork
Publication Date
2019-08
Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.116, no.35, pp.17438 - 17443
Publisher
NATL ACAD SCIENCES
Abstract
© 2019 National Academy of Sciences. All rights reserved.Defects in DNA repair give rise to genomic instability, leading to neoplasia. Cancer cells defective in one DNA repair pathway can become reliant on remaining repair pathways for survival and proliferation. This attribute of cancer cells can be exploited therapeutically, by inhibiting the remaining repair pathway, a process termed synthetic lethality. This process underlies the mechanism of the Poly-ADP ribose polymerase-1 (PARP1) inhibitors in clinical use, which target BRCA1 deficient cancers, which is indispensable for homologous recombination (HR) DNA repair. HR is the major repair pathway for stressed replication forks, but when BRCA1 is deficient, stressed forks are repaired by back-up pathways such as alternative nonhomologous end-joining (aNHEJ). Unlike HR, aNHEJ is nonconservative, and can mediate chromosomal translocations. In this study we have found that miR223-3p decreases expression of PARP1, CtIP, and Pso4, each of which are aNHEJ components. In most cells, high levels of microRNA (miR) 223-3p repress aNHEJ, decreasing the risk of chromosomal translocations. Deletion of the miR223 locus in mice increases PARP1 levels in hematopoietic cells and enhances their risk of unprovoked chromosomal translocations. We also discovered that cancer cells deficient in BRCA1 or its obligate partner BRCA1-Associated Protein-1 (BAP1) routinely repress miR223-3p to permit repair of stressed replication forks via aNHEJ. Reconstituting the expression of miR223-3p in BRCA1- and BAP1-deficient cancer cells results in reduced repair of stressed replication forks and synthetic lethality. Thus, miR223-3p is a negative regulator of the aNHEJ DNA repair and represents a therapeutic pathway for BRCA1- or BAP1-deficient cancers
URI
https://pr.ibs.re.kr/handle/8788114/6364
DOI
10.1073/pnas.1903150116
ISSN
0027-8424
Appears in Collections:
Center for Genomic Integrity(유전체 항상성 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
NAS_2019_MiR223-3p promotes synthetic lethality in BRCA1-deficient cancers.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse