Regulation of PCNA cycling on replicating DNA by RFC and RFC-like complexes

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Title
Regulation of PCNA cycling on replicating DNA by RFC and RFC-like complexes
Author(s)
Mi-Sun Kang; Eunjin Ryu; Seung-Won Lee; Jieun Park; Na Young Ha; Jae Sun Ra; Yeong Jae Kim; Jinwoo Kim; Mohamed Abdel-Rahman; Su Hyung Park; Kyoo-young Lee; Hajin Kim; Sukhyun Kang; Kyungjae Myung
Publication Date
2019-06
Journal
NATURE COMMUNICATIONS, v.10, no., pp.2420 -
Publisher
NATURE PUBLISHING GROUP
Abstract
Replication-Factor-C (RFC) and RFC-like complexes (RLCs) mediate chromatin engagement of the proliferating cell nuclear antigen (PCNA). It remains controversial how RFC and RLCs cooperate to regulate PCNA loading and unloading. Here, we show the distinct PCNA loading or unloading activity of each clamp loader. ATAD5-RLC possesses the potent PCNA unloading activity. ATPase motif and collar domain of ATAD5 are crucial for the unloading activity. DNA structures did not affect PCNA unloading activity of ATAD5-RLC. ATAD5-RLC could unload ubiquitinated PCNA. Through single molecule measurements, we reveal that ATAD5-RLC unloaded PCNA through one intermediate state before ATP hydrolysis. RFC loaded PCNA through two intermediate states on DNA, separated by ATP hydrolysis. Replication proteins such as Feni could inhibit the PCNA unloading activity of Elg1-RLC, a yeast homolog of ATAD5-RLC in vitro. Our findings provide molecular insights into how PCNA is released from chromatin to finalize DNA replication/repair
URI
https://pr.ibs.re.kr/handle/8788114/6164
ISSN
2041-1723
Appears in Collections:
Center for Genomic Integrity(유전체 항상성 연구단) > Journal Papers (저널논문)
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