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혈관 연구단
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Neutrophils disturb pulmonary microcirculation in sepsis-induced acute lung injury

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Title
Neutrophils disturb pulmonary microcirculation in sepsis-induced acute lung injury
Author(s)
Park I.; Kim M.; Choe K.; Song E.; Seo H.; Hwang Y.; Ahn J.; Lee S.-H.; Lee J.H.; Jo Y.H.; Kim K.; Gou Young Koh; Kim P.
Publication Date
2019-03
Journal
EUROPEAN RESPIRATORY JOURNAL, v.53, no.3, pp.1800786 -
Publisher
EUROPEAN RESPIRATORY SOC JOURNALS LTD
Abstract
The lung is highly vulnerable during sepsis, yet its functional deterioration accompanied by disturbances in the pulmonary microcirculation is poorly understood. This study aimed to investigate how the pulmonary microcirculation is distorted in sepsis-induced acute lung injury (ALI) and reveal the underlying cellular pathophysiologic mechanism. Using a custom-made intravital lung microscopic imaging system in a murine model of sepsis-induced ALI, we achieved direct real-time visualisation of the pulmonary microcirculation and circulating cells in vivo. We derived the functional capillary ratio (FCR) as a quantitative parameter for assessing the fraction of functional microvasculature in the pulmonary microcirculation and dead space. We identified that the FCR rapidly decreases in the early stage of sepsis-induced ALI. The intravital imaging revealed that this decrease resulted from the generation of dead space, which was induced by prolonged neutrophil entrapment within the capillaries. We further showed that the neutrophils had an extended sequestration time and an arrest-like dynamic behaviour, both of which triggered neutrophil aggregates inside the capillaries and arterioles. Finally, we found that Mac-1 (CD11b/CD18) was upregulated in the sequestered neutrophils and that a Mac-1 inhibitor restored the FCR and improved hypoxaemia. Using the intravital lung imaging system, we observed that Mac-1-upregulated neutrophil aggregates led to the generation of dead space in the pulmonary microcirculation that was recovered by a Mac-1 inhibitor in sepsis-induced ALI. Copyright ©ERS 2019
URI
https://pr.ibs.re.kr/handle/8788114/5883
ISSN
0903-1936
Appears in Collections:
Center for Vascular Research(혈관 연구단) > Journal Papers (저널논문)
Files in This Item:
ERJ-00786-2018.pdfDownload

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