Spatial regulation of ABCG25, an ABA exporter, is an important component of the mechanism controlling cellular aba levels

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Title
Spatial regulation of ABCG25, an ABA exporter, is an important component of the mechanism controlling cellular aba levels
Author(s)
Park Y.; Xu Z.-Y.; Kim S.Y.; Lee J.; Choi B.; Lee J.; Hyeran Kim; Hee-Jung Sim; Hwang I.
Publication Date
2016-10
Journal
PLANT CELL, v.28, no.10, pp.2528 - 2544
Publisher
AMER SOC PLANT BIOLOGISTS
Abstract
The phytohormone abscisic acid (ABA) plays crucial roles in various physiological processes, including responses to abiotic stresses, in plants. Recently, multiple ABA transporters were identified. The loss-of-function and gain-of-function mutants of these transporters show altered ABA sensitivity and stomata regulation, highlighting the importance of ABA transporters in ABA-mediated processes. However, how the activity of these transporters is regulated remains elusive. Here, we show that spatial regulation of ATP BINDING CASETTE G25 (ABCG25), an ABA exporter, is an important mechanism controlling its activity. ABCG25, as a soluble green fluorescent protein (sGFP) fusion, was subject to posttranslational regulation via clathrin-dependent and adaptor protein complex-2-dependent endocytosis followed by trafficking to the vacuole. The levels of sGFP:ABCG25 at the plasma membrane (PM) were regulated by abiotic stresses and exogenously applied ABA; PMlocalized sGFP:ABCG25 decreased under abiotic stress conditions via activation of endocytosis in an ABA-independent manner, but increased upon application of exogenous ABA via activation of recycling from early endosomes in an ABAdependent manner. Based on these findings, we propose that the spatial regulation of ABCG25 is an important component of the mechanism by which plants fine-tune cellular ABA levels according to cellular and environmental conditions. © 2016 American Society of Plant Biologists. All rights reserved
URI
https://pr.ibs.re.kr/handle/8788114/5805
ISSN
1040-4651
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > Journal Papers (저널논문)
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