Fusion guide RNAs for orthogonal gene manipulation with Cas9 and Cpf1

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Title
Fusion guide RNAs for orthogonal gene manipulation with Cas9 and Cpf1
Author(s)
Kweon, J; Jang, AH; Da-eun Kim; Yang, JW; Yoon, M; Shin, HR; Kim, JS; Kim, Y
Publication Date
2017-11
Journal
NATURE COMMUNICATIONS, v.8, no., pp.1723 -
Publisher
NATURE PUBLISHING GROUP
Abstract
The bacteria-derived clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems are powerful tools for genome engineering. Recently, in addition to Cas protein engineering, the improvement of guide RNAs are also performed, contributing to broadening the research area of CRISPR-Cas9 systems. Here we develop a fusion guide RNA (fgRNA) that functions with both Cas9 and Cpf1 proteins to induce mutations in human cells. Furthermore, we demonstrate that fgRNAs can be used in multiplex genome editing and orthogonal genome manipulation with two types of Cas proteins. Our results show that fgRNAs can be used as a tool for performing multiple gene manipulations © The Author(s) 2018
URI
https://pr.ibs.re.kr/handle/8788114/5799
ISSN
2041-1723
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > Journal Papers (저널논문)
Files in This Item:
성과추가_1711_사사X_김진수_NatureComm.pdfDownload

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