Deuterium-Free, Three-Plexed Peptide Diethylation for Highly Accurate Quantitative Proteomics

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Title
Deuterium-Free, Three-Plexed Peptide Diethylation for Highly Accurate Quantitative Proteomics
Author(s)
Jung J.; Kyowon Jeong; Yeon Choi; Sun Ah Kim; Hyunjoon Kim; Lee J.W.; V. Narry Kim; Kim K.P.; Jong-Seo Kim
Publication Date
2019-03
Journal
JOURNAL OF PROTEOME RESEARCH, v.18, no.3, pp.1078 - 1087
Publisher
AMER CHEMICAL SOC
Abstract
The deuterium, a frequently used stable isotope in isotopic labeling for quantitative proteomics, could deteriorate the accuracy and precision of proteome quantification owing to the retention time shift of deuterated peptides from the hydrogenated counterpart. We introduce a novel three-plexed peptide diethylation using only 13C isotopologues of acetaldehyde and demonstrate that the accuracy and precision of our method in proteome quantification are significantly superior to the conventional deuterium-based dimethylation labeling in both a single-shot and multidimensional LC-MS/MS analysis of the HeLa proteome. Furthermore, in time-resolved profiling of Xenopus laevis early embryogenesis, our 3-plexed diethylation outperformed isobaric labeling approaches in terms of the quantification accuracy or the number of protein identifications, generating more than two times more differentially expressed proteins. Our cost-effective and highly accurate 3-plexed diethylation method could contribute to various types of quantitative proteomics applications in which three of multiplexity would be sufficient. Copyright © 2019 American Chemical Society
URI
https://pr.ibs.re.kr/handle/8788114/5681
ISSN
1535-3893
Appears in Collections:
Center for RNA Research(RNA 연구단) > Journal Papers (저널논문)
Files in This Item:
acs.jproteome.8b00775.pdfDownload

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