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유전체 교정 연구단
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Multi-color fluorescence of pNIPAM-Based nanogels modulated by dual stimuli-responsive FRET processes

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Title
Multi-color fluorescence of pNIPAM-Based nanogels modulated by dual stimuli-responsive FRET processes
Author(s)
Seung Hwan Lee; Bui H.T.; Vales T.P.; Cho S.; Kim H.-J.
Publication Date
2017-10
Journal
DYES AND PIGMENTS, v.145, no., pp.216 - 221
Publisher
ELSEVIER SCI LTD
Abstract
We report the modulation of the dual photo/thermo-responsive multicolor fluorescence from poly(N-isopropyl acrylate) pNIPAM-based nanogels. The thermo-responsive pNIPAM-crosslinked matrix was covalently copolymerized with nitrobenzoxadiazole (NBD) monomers as a Förster resonance energy transfer (FRET) donor and electrostatically conjugated with photochromic spiropyran (SP) dyes as a FRET acceptor. The pristine nanogel emits green fluorescence derived from the NBD moieties; however, the irradiation of the nanogels with UV light led to the transformation of the ring-closed colorless SP moieties to the ring-opened coral-red merocyanine (MC) moieties, resulting in light-pink fluorescent nanogels. Notably, at higher temperatures, the fluorescence of the nanogels was red-shifted and enhanced by the more efficient FRET process. The nanogels were well-distributed in the cellular cytoplasm and showed excellent photochromic function in the subcellular environment for applications as photo-switchable bio-imaging agents and nanothermometers. © 2017 Elsevier Lt
URI
https://pr.ibs.re.kr/handle/8788114/5615
ISSN
0143-7208
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Center for Genome Engineering(유전체 교정 연구단) > Journal Papers (저널논문)
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