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나노물질 및 화학반응 연구단
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Regulation of protein structural changes by incorporation of a small-molecule linker

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Title
Regulation of protein structural changes by incorporation of a small-molecule linker
Author(s)
Youngmin Kim; Cheolhee Yang; TaeWu Kim; Kamatchi Thamilselvan; Yonggwan Kim; Hyotcherl Ihee
Publication Date
2018-12
Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.19, no.12, pp.3714 -
Publisher
MDPI AG
Abstract
Proteins have the potential to serve as nanomachines with well-controlled structural movements, and artificial control of their conformational changes is highly desirable for successful applications exploiting their dynamic structural characteristics. Here, we demonstrate an experimental approach for regulating the degree of conformational change in proteins by incorporating a small-molecule linker into a well-known photosensitive protein, photoactive yellow protein (PYP), which is sensitized by blue light and undergoes a photo-induced N-terminal protrusion coupled with chromophore-isomerization-triggered conformational changes. Specifically, we introduced thiol groups into specific sites of PYP through site-directed mutagenesis and then covalently conjugated a small-molecule linker into these sites, with the expectation that the linker is likely to constrain the structural changes associated with the attached positions. To investigate the structural dynamics of PYP incorporated with the small-molecule linker (SML-PYP), we employed the combination of small-angle X-ray scattering (SAXS), transient absorption (TA) spectroscopy and experiment-restrained rigid-body molecular dynamics (MD) simulation. Our results show that SML-PYP exhibits much reduced structural changes during photo-induced signaling as compared to wild-type PYP. This demonstrates that incorporating an external molecular linker can limit photo-induced structural dynamics of the protein and may be used as a strategy for fine control of protein structural dynamics in nanomachines. © 2018 by the authors. Licensee MDPI, Basel, Switzerland
URI
https://pr.ibs.re.kr/handle/8788114/5317
ISSN
1661-6596
Appears in Collections:
Center for Nanomaterials and Chemical Reactions(나노물질 및 화학반응 연구단) > Journal Papers (저널논문)
Files in This Item:
Int. J. Mol. Sci. 2018, 19, 3714.pdfDownload

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