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Production of exopolysaccharides by Lactococcus lactis subsp. cremoris MG1363 expressing the eps gene clusters from two strains of Lactobacillus rhamnosus

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Title
Production of exopolysaccharides by Lactococcus lactis subsp. cremoris MG1363 expressing the eps gene clusters from two strains of Lactobacillus rhamnosus
Author(s)
Hye-ji Kang; Gisele Lapointe
Publication Date
2018-06
Journal
Korean Journal of Microbiology and Biotechnology, v.46, no.2, pp.91 - 101
Publisher
Korean Society for Applied Microbiology
Abstract
The aim of this study was to transfer the 18.5 kb gene clusters coding for 17 genes from Lactobacillus rhamnosus to Lactococcus lactis subsp. cremoris MG1363 in order to determine the effect of host on exopolysaccharide (EPS) production and to provide a model for studying the phosphorylation of proteins which are proposed to be involved in EPS polymerization. Lactobacillus rhamnosus RW-9595M and ATCC 9595 have 99% identical operons coding for EPS biosynthesis, produced different amounts of EPS (543 vs 108 mg/l). L. lactis subsp. cremoris MG1363 transformed with the operons from RW-9595M and ATCC 9595 respectively, produced 326 and 302 mg/l EPS in M17 containing 0.5% glucose. The tyrosine protein kinase transmembrane modulator (Wzd) was proposed to participate in regulating chain elongation of EPS polymers by interacting with the tyrosine protein kinase Wze. While Wzd was found in phosphorylated form in the presence of the phosphorylated kinase (Wze), no phosphorylated proteins were detected when all nine tyrosines of Wzd were mutated to phenylalanine. Lactococcus lactis subsp. cremoris could produce higher amounts of EPS than other EPS-producing lactococci when expressing genes from L. rhamnosus. Phosphorylated Wzd was essential for the phosphorylation of Wze when expressed in vivo. ? 2018, The Korean Society for Microbiology and Biotechnology
URI
https://pr.ibs.re.kr/handle/8788114/5269
ISSN
1598-642X
Appears in Collections:
Academy of Immunology and Microbiology(면역 미생물 공생 연구단) > Journal Papers (저널논문)
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