Millisecond cellular labelling in situ with two-photon photoconversion

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Title
Millisecond cellular labelling in situ with two-photon photoconversion
Author(s)
Sheldon J. J. Kwok; Yongjae Jo; Harvey H. Lin; Myunghwan Choi; Seok-Hyun Yun
Publication Date
2018-07
Journal
Biomedical Optics Express, v.9, no.7, pp.3067 - 3077
Publisher
OPTICAL SOC AMER
Abstract
In situ labeling of cells within living biological tissues using photoconversion has provided valuable information on cellular physiology in their natural environments. However, current photoconvertible probes typically require seconds to minutes of light exposure, limiting their uses in rapid biological processes such as intracellular diffusion and circulating cells. Here, we report that two-photon photoconversion of cyanine-based dyes offers unprecedentedly rapid photoconversion down to millisecond timescales per cell. We demonstrate potential biological applications including measuring intracellular diffusion kinetics in a spinal nerve, labeling of rapidly flowing cells in a microfluidic channel, and photoconversion of a circulating cell in vivo. © 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
URI
https://pr.ibs.re.kr/handle/8788114/5248
ISSN
2156-7085
Appears in Collections:
Center for Neuroscience Imaging Research (뇌과학 이미징 연구단) > Journal Papers (저널논문)
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