Mouse fMRI under ketamine and xylazine anesthesia: Robust contralateral somatosensory cortex activation in response to forepaw stimulation

Cited 0 time in webofscience Cited 0 time in scopus
8 Viewed 1 Downloaded
Title
Mouse fMRI under ketamine and xylazine anesthesia: Robust contralateral somatosensory cortex activation in response to forepaw stimulation
Author(s)
Hyun-ji shim; Won Beom Jung; Felix Schlegel; Joonsung Lee; Sangwoo Kim; Jungryun Lee; Seong-Gi Kim
Publication Date
2018-08
Journal
NEUROIMAGE, v.177, no., pp.30 - 44
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Abstract
Mouse fMRI is critically useful to investigate functions of mouse models. Until now, the somatosensory-evoked responses in anesthetized mice are often widespread and inconsistent across reports. Here, we adopted a ketamine and xylazine mixture for mouse fMRI, which is relatively new anesthetics in fMRI experiments. Forepaw stimulation frequency was optimized using cerebral blood volume (CBV)-weighted optical imaging (n = 11) and blood-oxygenation-level dependent (BOLD) fMRI with a gradient-echo time of 16 ms at 9.4 T, and 4 Hz stimulation with 0.5 ms and 0.5 mA pulses induced the highest hemodynamic response. For 20-s 4-Hz unilateral forepaw stimulation, localized BOLD activity was consistently found in the contralateral primary forelimb somatosensory cortex (S1FL), while no significant change was observed in the ipsilateral S1FL. The mean magnitude was 1.44 +/- 0.20% SEM (n = 9) in the contralateral S1FL and 0.69 +/- 0.10% in the contralateral thalamus. The variability of evoked fMRI responses across sessions was investigated by comparing with resting state fMRI (rsfMRI) functional connectivity (FC). Evoked responses in S1FL were correlated positively with rsfMRI FC between bilateral S1FL (r = 0.63 to 0.69) and negatively with FC between S1FL and the anterior cingulate cortex (r = -0.50 to -0.57), suggesting that rsfMRI FC is a good index of the evoked fMRI response and anesthetized animal condition. Finally, three weekly fMRI scans were performed in 5 mice, and localized activity was reproducibly observed in S1FL, with a success rate of 70-95%. In summary, our developed fMRI protocol can be used for mapping functions of mouse models.(NeuroImage 2018)(c) 2018 Elsevier Inc. All rights reserved.
URI
https://pr.ibs.re.kr/handle/8788114/5239
ISSN
1053-8119
Appears in Collections:
Center for Neuroscience Imaging Research (뇌과학 이미징 연구단) > Journal Papers (저널논문)
Files in This Item:
Mouse fMRI under ketamine and xylazine anesthesia Robust contralateral_김성기.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse