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면역 미생물 공생 연구단
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Two-photon microscopy of Paneth cells in the small intestine of live mice

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Title
Two-photon microscopy of Paneth cells in the small intestine of live mice
Author(s)
Won Hyuk Jang; Areum Park; Taejun Wang; Chan Johng Kim; Hoonchul Chang; Bo-Gie Yang; Myoung Joon Kim; Seung-Jae Myung; Sin-Hyeog Im; Myoung Ho Jang; You-Me Kim; Ki Hean Kim
Publication Date
2018-09
Journal
SCIENTIFIC REPORTS, v.8, no.1, pp.14174 -
Publisher
NATURE PUBLISHING GROUP
Abstract
Paneth cells are one of the principal epithelial cell types in the small intestine, located at the base of intestinal crypts. Paneth cells play key roles in intestinal host-microbe homeostasis via granule secretion, and their dysfunction is implicated in pathogenesis of several diseases including Crohn's disease. Despite their physiological importance, study of Paneth cells has been hampered by the limited accessibility and lack of labeling methods. In this study, we developed a simple in vivo imaging method of Paneth cells in the intact mouse small intestine by using moxifloxacin and two-photon microscopy (TPM). Moxifloxacin, an FDA-approved antibiotic, was used for labeling cells and its fluorescence was strongly observed in Paneth cell granules by TPM. Moxifloxacin labeling of Paneth cell granules was confirmed by molecular counterstaining. Comparison of Paneth cells in wild type, genetically obese (ob/ob), and germ-free (GF) mice showed different granule distribution. Furthermore, Paneth cell degranulation was observed in vivo. Our study suggests that TPM with moxifloxacin labeling can serve as a useful tool for studying Paneth cell biology and related diseases © The Author(s) 2018
URI
https://pr.ibs.re.kr/handle/8788114/5173
ISSN
2045-2322
Appears in Collections:
Academy of Immunology and Microbiology(면역 미생물 공생 연구단) > Journal Papers (저널논문)
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