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Improving collection efficiency in two-photon endoscopy with reflective waveguiding

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Title
Improving collection efficiency in two-photon endoscopy with reflective waveguiding
Author(s)
Jisoo Han; Sungho Lee; Pyonggang Choi; Jiamin Wu; Keunhyung Lee; Qionghai Dai; Jinhyung Park; Kyung-Bok Lee; Myunghwan Choi
Publication Date
2018-11
Journal
OPTICS EXPRESS, v.26, no.25, pp.32365 - 32373
Publisher
OPTICAL SOC AMER
Abstract
Two-photon endoscopy based on a gradient-index lens has been widely utilized for studying cellular behaviors in deep-lying tissues with minimal invasiveness in vivo. Although the efficient collection of emitted light is critical to attain high-contrast spatiotemporal information, the intrinsic low numerical aperture of the endoscopic probe poses a physical limitation. We report a simple solution to overcome this limit by incorporating a reflective waveguide ensheathing the endoscopic probe, which improves the collection efficiency by approximately two-fold. We describe its principle, fabrication procedure, optical characterization, and utilities in biological tissues. © 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
URI
https://pr.ibs.re.kr/handle/8788114/5091
DOI
10.1364/OE.26.032365
ISSN
1094-4087
Appears in Collections:
Center for Neuroscience Imaging Research (뇌과학 이미징 연구단) > 1. Journal Papers (저널논문)
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