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식물 노화·수명 연구단
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Establishment of stably expandable induced myogenic stem cells by four transcription factors

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Title
Establishment of stably expandable induced myogenic stem cells by four transcription factors
Author(s)
Eun-Joo Lee; Minhyung Kim; Yong Deuk Kim; Myung-Jin Chung; Ahmed Elfadl; H. M. Arif Ulah; Dongsu Park; Sunray Lee; Hyun-Sook Park; Tae-Hwan Kim; Daehee Hwang; Kyu-Shik Jeong
Publication Date
2018-11
Journal
CELL DEATH & DISEASE, v.9, no.11, pp.1092 -
Publisher
NATURE PUBLISHING GROUP
Abstract
Life-long regeneration of healthy muscle by cell transplantation is an ideal therapy for patients with degenerative muscle diseases. Yet, obtaining muscle stem cells from patients is very limited due to their exhaustion in disease condition. Thus, development of a method to obtain healthy myogenic stem cells is required. Here, we showed that the four transcription factors, Six1, Eya1, Esrrb, and Pax3, converts fibroblasts into induced myogenic stem cells (iMSCs). The iMSCs showed effective differentiation into multinucleated myotubes and also higher proliferation capacity than muscle derived stem cells both in vitro and in vivo. The iMSCs do not lose their proliferation capacity though the passaging number is increased. We further isolated CD106-negative and α7-integrin-positive iMSCs (sort-iMSCs) showing higher myogenic differentiation capacity than iMSCs. Moreover, genome-wide transcriptomic analysis of iMSCs and sort-iMSCs, followed by network analysis, revealed the genes and signaling pathways associated with enhanced proliferation and differentiation capacity of iMSCs and sort-iMSCs, respectively. The stably expandable iMSCs provide a new source for drug screening and muscle regenerative therapy for muscle wasting disease. © The Author(s) 2018
URI
https://pr.ibs.re.kr/handle/8788114/5049
ISSN
2041-4889
Appears in Collections:
Center for Plant Aging Research (식물 노화·수명 연구단) > Journal Papers (저널논문)
Files in This Item:
Cell Death & Diseasevolume 9, 1092 (2018).pdfDownload

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