High-throughput chemical screening to discover new modulators of microRNA expression in living cells by using graphene-based biosensor

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Title
High-throughput chemical screening to discover new modulators of microRNA expression in living cells by using graphene-based biosensor
Author(s)
Soo-Ryoon Ryoo; Yeajee Yim; Young-Kwan Kim; Il-Soo Par; Hee-Kyung Na; Jieon Lee; Hongje Jang; CheolheeWon; Sungwoo Hong; Sung-Yon Kim; Noo Li Jeon; Joon Myong Song; Dal-Hee Min
Publication Date
2018-07
Journal
SCIENTIFIC REPORTS, v.8, no., pp.11413 -
Publisher
NATURE PUBLISHING GROUP
Abstract
MicroRNAs (miRNAs) are important regulatory RNAs that control gene expression in various biological processes. Therefore, control over the disease-related miRNA expression is important both for basic research and for a new class of therapeutic modality to treat serious diseases such as cancer. Here, we present a high-throughput screening strategy to identify small molecules that modulate miRNA expression in living cells. The screen enables simultaneous monitoring of the phenotypic cellular changes associated with the miRNA expression by measuring quantitative fluorescent signals corresponding to target miRNA level in living cells based on a novel biosensor composed of peptide nucleic acid and nano-sized graphene oxide. In this study, the biosensor based cellular screening of 967 compounds (including FDA-approved drugs, enzyme inhibitors, agonists, and antagonists) in cells identified four different classes of small molecules consisting of (i) 70 compounds that suppress both miRNA-21 (miR-21) expression and cell proliferation, (ii) 65 compounds that enhance miR-21 expression and reduce cell proliferation, (iii) 2 compounds that suppress miR-21 expression and increase cell proliferation, and (iv) 21 compounds that enhance both miR-21 expression and cell proliferation. We further investigated the hit compounds to correlate cell morphology changes and cell migration ability with decreased expression of miR-21 © Te Author(s) 2018
URI
https://pr.ibs.re.kr/handle/8788114/4962
ISSN
2045-2322
Appears in Collections:
Center for Catalytic Hydrocarbon Functionalizations(분자활성 촉매반응 연구단) > Journal Papers (저널논문)
Center for RNA Research(RNA 연구단) > Journal Papers (저널논문)
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