Measurements of complex refractive index change of photoactive yellow protein over a wide wavelength range using hyperspectral quantitative phase imaging

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Title
Measurements of complex refractive index change of photoactive yellow protein over a wide wavelength range using hyperspectral quantitative phase imaging
Author(s)
KyeReh Lee; Youngmin Kim; JaeHwang Jung; Hyotcherl Ihee; YongKeun Park
Publication Date
2018-02
Journal
SCIENTIFIC REPORTS, v.8, no.1, pp.3064 -
Publisher
NATURE PUBLISHING GROUP
Abstract
A novel optical holographic technique is presented to simultaneously measure both the real and imaginary components of the complex refractive index (CRI) of a protein solution over a wide visible wavelength range. Quantitative phase imaging was employed to precisely measure the optical field transmitted from a protein solution, from which the CRIs of the protein solution were retrieved using the Fourier light scattering technique. Using this method, we characterized the CRIs of the two dominant structural states of a photoactive yellow protein solution over a broad wavelength range (461-582 nm). The significant CRI deviation between the two structural states was quantified and analysed. The results of both states show the similar overall shape of the expected rRI obtained from the Kramers-Kronig relations. © 2018 The Author(s)
URI
https://pr.ibs.re.kr/handle/8788114/4666
ISSN
2045-2322
Appears in Collections:
Center for Nanomaterials and Chemical Reactions(나노물질 및 화학반응 연구단) > Journal Papers (저널논문)
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